TY - JOUR
T1 - Cloning and heterologous expression of the genes encoding nonspecific electron transport components for a cytochrome P450 system of Saccharopolyspora erythraea involved in erythromycin production
AU - Zotchev, Sergey B.
AU - Hutchinson, C. Richard
PY - 1995/4/14
Y1 - 1995/4/14
N2 - The forA gene encoding a protein that can function as a NADH: ferredoxin oxidoreductase (For) has been cloned from Saccharopolyspora erythraea, the erythromycin A (ErA) producer. In a previous study For protein, together with the FdxA ferredoxin from the same organism, was shown to be able to reconstitute the cytochrome P450 system responsible for the hydroxylation of 6-deoxyerythronolide B, an intermediate of ErA biosynthesis. Nucleotide sequence data suggest that the cloned forA gene codes for For, the putative pyruvate dehydrogenase component, dihydrolipoamide dehydrogenase, or its close homolog. Overexpression of forA appeared to be toxic to Escherichia coli.
AB - The forA gene encoding a protein that can function as a NADH: ferredoxin oxidoreductase (For) has been cloned from Saccharopolyspora erythraea, the erythromycin A (ErA) producer. In a previous study For protein, together with the FdxA ferredoxin from the same organism, was shown to be able to reconstitute the cytochrome P450 system responsible for the hydroxylation of 6-deoxyerythronolide B, an intermediate of ErA biosynthesis. Nucleotide sequence data suggest that the cloned forA gene codes for For, the putative pyruvate dehydrogenase component, dihydrolipoamide dehydrogenase, or its close homolog. Overexpression of forA appeared to be toxic to Escherichia coli.
KW - Antibiotic biosynthesis
KW - ferredoxin
KW - oxidoreductase
UR - http://www.scopus.com/inward/record.url?scp=0028915041&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(95)00012-U
DO - 10.1016/0378-1119(95)00012-U
M3 - Article
C2 - 7737500
AN - SCOPUS:0028915041
SN - 0378-1119
VL - 156
SP - 101
EP - 106
JO - Gene
JF - Gene
IS - 1
ER -