Combined Chemisorption and Complexation Generate siRNA Nanocarriers with Biophysics Optimized for Efficient Gene Knockdown and Air-Blood Barrier Crossing

Alexander Taschauer; Wolfram Polzer; Stefan Poeschl; Slavica Metz; Nathalie Tepe; Simon Decker; Norbert Cyran; Julia Scholda; Julia Maier; Hermann Bloss; Martina Anton; Thilo Hofmann; Manfred Ogris; Haider Sami

doi:10.1021/acsami.0c06608

Combined Chemisorption and Complexation Generate siRNA Nanocarriers with Biophysics Optimized for Efficient Gene Knockdown and Air-Blood Barrier Crossing

Alexander Taschauer, Wolfram Polzer, Stefan Poeschl, Slavica Metz, Nathalie Tepe, Simon Decker, Norbert Cyran, Julia Scholda, Julia Maier, Hermann Bloss, Martina Anton, Thilo Hofmann, Manfred Ogris (Corresponding author), Haider Sami (Corresponding author)

Publications: Contribution to journal › Article › Peer Reviewed

Original language	English
Pages (from-to)	30095-30111
Number of pages	17
Journal	ACS applied materials & interfaces
Volume	12
Issue number	27
DOIs	https://doi.org/10.1021/acsami.0c06608
Publication status	Published - 9 Jun 2020

Funding

The research leading to these results has received support from the Innovative Medicines Initiative Joint Undertaking under grant agreement no. 115363, resources of which are composed of financial contribution from the European Union's Seventh Framework Programme (FP7/2007-2013) and EFPIA companies in kind contribution and the NANOBILD (Development and application of nanomaterials for multimodal medical imaging), an infrastructure grant of the Federal Ministry of Science, Research and Economy (BMBWF) from Austria. Antonia Geyer and Theresa Kittelmann are gratefully acknowledged for help in setting up the initial in vivo measurements. The oligonucleotides used in this work were supplied by GSK (GSK Medicines Research Centre, Stevenage, UK; synthesis by Glynn Williams and Jonathan Northall) as part of their contribution to the EU-funded IMI project COMPACT.

Austrian Fields of Science 2012

105906 Environmental geosciences
106022 Microbiology

Keywords

gold nanoparticle
linear polyethylenimine
siRNA knockdown
pulmonary delivery
intratracheal
tomograpluc optical imaging
biodistribution
BY-LAYER NANOPARTICLES
GOLD NANOPARTICLES
INTRACELLULAR TRAFFICKING
SYSTEMIC DELIVERY
CELLULAR UPTAKE
CLINICAL-TRIAL
POLYETHYLENIMINE
POLYPLEXES
DNA
OLIGONUCLEOTIDE
tomographic optical imaging

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10.1021/acsami.0c06608Licence: CC BY 4.0

https://phaidra.univie.ac.at/o:2094683Licence: CC BY 4.0

Cite this

Taschauer, A., Polzer, W., Poeschl, S., Metz, S., Tepe, N., Decker, S., Cyran, N., Scholda, J., Maier, J., Bloss, H., Anton, M., Hofmann, T., Ogris, M., & Sami, H. (2020). Combined Chemisorption and Complexation Generate siRNA Nanocarriers with Biophysics Optimized for Efficient Gene Knockdown and Air-Blood Barrier Crossing. ACS applied materials & interfaces, 12(27), 30095-30111. https://doi.org/10.1021/acsami.0c06608

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title = "Combined Chemisorption and Complexation Generate siRNA Nanocarriers with Biophysics Optimized for Efficient Gene Knockdown and Air-Blood Barrier Crossing",

keywords = "gold nanoparticle, linear polyethylenimine, siRNA knockdown, pulmonary delivery, intratracheal, tomograpluc optical imaging, biodistribution, BY-LAYER NANOPARTICLES, GOLD NANOPARTICLES, INTRACELLULAR TRAFFICKING, SYSTEMIC DELIVERY, CELLULAR UPTAKE, CLINICAL-TRIAL, POLYETHYLENIMINE, POLYPLEXES, DNA, OLIGONUCLEOTIDE, tomographic optical imaging",

author = "Alexander Taschauer and Wolfram Polzer and Stefan Poeschl and Slavica Metz and Nathalie Tepe and Simon Decker and Norbert Cyran and Julia Scholda and Julia Maier and Hermann Bloss and Martina Anton and Thilo Hofmann and Manfred Ogris and Haider Sami",

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year = "2020",

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language = "English",

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Taschauer, A, Polzer, W, Poeschl, S, Metz, S, Tepe, N, Decker, S , Cyran, N, Scholda, J, Maier, J, Bloss, H, Anton, M, Hofmann, T , Ogris, M & Sami, H 2020, 'Combined Chemisorption and Complexation Generate siRNA Nanocarriers with Biophysics Optimized for Efficient Gene Knockdown and Air-Blood Barrier Crossing', ACS applied materials & interfaces, vol. 12, no. 27, pp. 30095-30111. https://doi.org/10.1021/acsami.0c06608

Combined Chemisorption and Complexation Generate siRNA Nanocarriers with Biophysics Optimized for Efficient Gene Knockdown and Air-Blood Barrier Crossing. / Taschauer, Alexander; Polzer, Wolfram; Poeschl, Stefan et al.
In: ACS applied materials & interfaces, Vol. 12, No. 27, 09.06.2020, p. 30095-30111.

Publications: Contribution to journal › Article › Peer Reviewed

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T1 - Combined Chemisorption and Complexation Generate siRNA Nanocarriers with Biophysics Optimized for Efficient Gene Knockdown and Air-Blood Barrier Crossing

AU - Taschauer, Alexander

AU - Polzer, Wolfram

AU - Poeschl, Stefan

AU - Metz, Slavica

AU - Tepe, Nathalie

AU - Decker, Simon

AU - Cyran, Norbert

AU - Scholda, Julia

AU - Maier, Julia

AU - Bloss, Hermann

AU - Anton, Martina

AU - Hofmann, Thilo

AU - Ogris, Manfred

AU - Sami, Haider

PY - 2020/6/9

Y1 - 2020/6/9

KW - gold nanoparticle

KW - linear polyethylenimine

KW - siRNA knockdown

KW - pulmonary delivery

KW - intratracheal

KW - tomograpluc optical imaging

KW - biodistribution

KW - BY-LAYER NANOPARTICLES

KW - GOLD NANOPARTICLES

KW - INTRACELLULAR TRAFFICKING

KW - SYSTEMIC DELIVERY

KW - CELLULAR UPTAKE

KW - CLINICAL-TRIAL

KW - POLYETHYLENIMINE

KW - POLYPLEXES

KW - DNA

KW - OLIGONUCLEOTIDE

KW - tomographic optical imaging

UR - https://www.scopus.com/pages/publications/85088210198

U2 - 10.1021/acsami.0c06608

DO - 10.1021/acsami.0c06608

M3 - Article

SN - 1944-8244

VL - 12

SP - 30095

EP - 30111

JO - ACS applied materials & interfaces

JF - ACS applied materials & interfaces

IS - 27

ER -

Combined Chemisorption and Complexation Generate siRNA Nanocarriers with Biophysics Optimized for Efficient Gene Knockdown and Air-Blood Barrier Crossing

Funding

Austrian Fields of Science 2012

Keywords

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