Abstract
Pearl millet is the fifth most important cereal crop worldwide and cultivated especially by small holder farmers in arid and semi-arid regions because of its drought and salt tolerance. The molecular mechanisms of drought stress tolerance in Pennisetum remain elusive. We have used a shotgun proteomics approach to investigate protein signatures from different tissues under drought and control conditions. Drought stressed plants showed significant changes in stomatal conductance and increased root growth compared to the control plants. Root, leaf and seed tissues were harvested and 2281 proteins were identified and quantified in total. Leaf tissue showed the largest number of significant changes (120), followed by roots (25) and seeds (10). Increased levels of root proteins involved in cell wall-, lipid-, secondary- and signaling metabolism and the concomitantly observed increased root length point to an impaired shoot–root communication under drought stress. The harvest index (HI) showed a significant reduction under drought stress. Proteins with a high correlation to the HI were identified using sparse partial least square (sPLS) analysis. Considering the importance of Pearl millet as a stress tolerant food crop, this study provides a first reference data set for future investigations of the underlying molecular mechanisms. Biological significance: Drought stress is the most limiting factor for plant growth and crop production worldwide. At the same time drought susceptible cereal crops are among the largest producers worldwide. In contrast, Pearl millet is a drought and salt tolerant cereal crop especially used in arid and semi-arid regions by small farmers. The multifactorial molecular mechanisms of this unique drought tolerance are not known. Here, we employ shotgun proteomics for a first characterization of the Pearl millet drought stress proteome. The experimental setup and the data set generated from this study reveal comprehensive physiological and proteomic responses of the drought stressed Pearl millet plants. Our study reveals statistically significant tissue-specific protein signatures during the adaptation to drought conditions. Thus, the work provides a first reference study of the drought stress proteome and related drought responsive proteins (DRP's) in Pearl millet.
| Original language | English |
|---|---|
| Pages (from-to) | 122-135 |
| Number of pages | 14 |
| Journal | Journal of Proteomics |
| Volume | 143 |
| DOIs | |
| Publication status | Published - 30 Jun 2016 |
Funding
We thank the European commission for the financial support of Palak Chaturvedi who is funded by the EU Marie-Curie International training network "Solanaceae pollen thermotolerance SPOT-ITN", grant agreement number 289220. We thank the EU Marie-Curie International training network "CALIPSO", grant number GA 2013-607-607, for financial support of Valentin Roustan. We thank the Austrian Science Fund (FWF) for financial support of Matthias Nagler by the Grant P 26342. We thank our gardeners and the glasshouse facility of the Department for Ecogenomics and Systems Biology for great support.
Austrian Fields of Science 2012
- 106037 Proteomics
- 106030 Plant ecology
Keywords
- Drought stress
- Plant productivity
- Global climate change
- Arid and semi-arid regions
- Multivariate statistics
- Tissue-specific
- Harvest index
- HEAT-SHOCK PROTEINS
- WATER-STRESS
- OSMOTIC ADJUSTMENT
- SOYBEAN ROOTS
- PLANT
- TOLERANCE
- WHEAT
- PHOTOSYNTHESIS
- EXPRESSION
- LEAVES
