Constraining and Modifying Peptides Using Pd-Mediated Cysteine Allylation

Julia Kriegesmann, Thomas Schlatzer, Kateryna Che, Claudia Altdorf, Susanne Huhmann, Hanspeter Kählig, Dennis Kurzbach, Rolf Breinbauer, Christian F.W. Becker (Corresponding author)

Publications: Contribution to journalArticlePeer Reviewed

Abstract

Over the past decades, several strategies for inducing and stabilizing secondary structure formation in peptides have been developed to increase their proteolytic stability and their binding affinity to specific interaction partners. Here, we report how our recently introduced chemoselective Pd-catalyzed cysteine allylation reaction can be extended to stapling and how the resulting alkene-containing staples themselves can be further modified to introduce additional probes into such stabilized peptides. The latter is demonstrated by introducing a fluorophore as well as a PEG moiety into different stapled peptides using bioorthogonal thiol-ene and Diels-Alder reactions. Furthermore, we investigated structural implications of our allyl staples when used to replace conformationally relevant disulfide bridges. To this end, we chose a selective binder of integrin α3β1 (LXY3), which is only active in its cyclic disulfide form. We replaced the disulfide bridge by different stapling reagents in order to increase stability and binding affinity towards integrin α3β1.

Original languageEnglish
Article numbere202300098
JournalChemBioChem
Volume24
Issue number13
DOIs
Publication statusPublished - 3 Jul 2023

Austrian Fields of Science 2012

  • 104004 Chemical biology
  • 106002 Biochemistry
  • 106023 Molecular biology

Keywords

  • allylation reaction
  • Diels-Alder reaction
  • peptide stapling
  • secondary peptide modification

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