Determination of deoxynivalenol in organic and conventional food and feed by sol-gel immunoaffinity chromatography and HPLC-UV detection

Christine Klinglmayr; Katharina Nöbauer; Ebrahim Razzazi-Fazeli; Margit Cichna-Markl

doi:10.1016/j.jchromb.2009.08.016

Determination of deoxynivalenol in organic and conventional food and feed by sol-gel immunoaffinity chromatography and HPLC-UV detection

Christine Klinglmayr, Katharina Nöbauer, Ebrahim Razzazi-Fazeli, Margit Cichna-Markl (Corresponding author)

Department of Analytical Chemistry

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Abstract

The paper describes the determination of deoxynivalenol (DON) in 55 wheat food and feed samples, 26 from conventional and 29 from organic production. Immunoaffinity columns prepared by entrapping anti-DON antibodies by the sol-gel method were used for sample clean-up. DON was quantified by high performance liquid chromatography (HPLC) and ultraviolet (UV) detection. In general, the incidence of DON contamination was rather low. In eight samples (14.5%) the DON concentration was above the LOQ (380 ng/g), in six samples (10.9%) DON was detected but could not be quantified (>LOD (200 ng/g), LOQ. The data indicate both a higher incidence of DON contamination and higher DON concentrations in food and feed samples from conventional than in those from organic production.

Original language	English
Pages (from-to)	187-193
Number of pages	7
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	878
Issue number	2
DOIs	https://doi.org/10.1016/j.jchromb.2009.08.016
Publication status	Published - 2010

Austrian Fields of Science 2012

104002 Analytical chemistry
104009 Food chemistry

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10.1016/j.jchromb.2009.08.016

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title = "Determination of deoxynivalenol in organic and conventional food and feed by sol-gel immunoaffinity chromatography and HPLC-UV detection",

abstract = "The paper describes the determination of deoxynivalenol (DON) in 55 wheat food and feed samples, 26 from conventional and 29 from organic production. Immunoaffinity columns prepared by entrapping anti-DON antibodies by the sol-gel method were used for sample clean-up. DON was quantified by high performance liquid chromatography (HPLC) and ultraviolet (UV) detection. In general, the incidence of DON contamination was rather low. In eight samples (14.5\%) the DON concentration was above the LOQ (380 ng/g), in six samples (10.9\%) DON was detected but could not be quantified (>LOD (200 ng/g), LOQ. The data indicate both a higher incidence of DON contamination and higher DON concentrations in food and feed samples from conventional than in those from organic production.",

author = "Christine Klinglmayr and Katharina N{\"o}bauer and Ebrahim Razzazi-Fazeli and Margit Cichna-Markl",

year = "2010",

doi = "10.1016/j.jchromb.2009.08.016",

language = "English",

volume = "878",

pages = "187--193",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

publisher = "Elsevier",

number = "2",

}

Klinglmayr, C, Nöbauer, K, Razzazi-Fazeli, E & Cichna-Markl, M 2010, 'Determination of deoxynivalenol in organic and conventional food and feed by sol-gel immunoaffinity chromatography and HPLC-UV detection', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 878, no. 2, pp. 187-193. https://doi.org/10.1016/j.jchromb.2009.08.016

Determination of deoxynivalenol in organic and conventional food and feed by sol-gel immunoaffinity chromatography and HPLC-UV detection. / Klinglmayr, Christine; Nöbauer, Katharina; Razzazi-Fazeli, Ebrahim et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 878, No. 2, 2010, p. 187-193.

Publications: Contribution to journal › Article › Peer Reviewed

TY - JOUR

T1 - Determination of deoxynivalenol in organic and conventional food and feed by sol-gel immunoaffinity chromatography and HPLC-UV detection

AU - Klinglmayr, Christine

AU - Nöbauer, Katharina

AU - Razzazi-Fazeli, Ebrahim

AU - Cichna-Markl, Margit

PY - 2010

Y1 - 2010

N2 - The paper describes the determination of deoxynivalenol (DON) in 55 wheat food and feed samples, 26 from conventional and 29 from organic production. Immunoaffinity columns prepared by entrapping anti-DON antibodies by the sol-gel method were used for sample clean-up. DON was quantified by high performance liquid chromatography (HPLC) and ultraviolet (UV) detection. In general, the incidence of DON contamination was rather low. In eight samples (14.5%) the DON concentration was above the LOQ (380 ng/g), in six samples (10.9%) DON was detected but could not be quantified (>LOD (200 ng/g), LOQ. The data indicate both a higher incidence of DON contamination and higher DON concentrations in food and feed samples from conventional than in those from organic production.

AB - The paper describes the determination of deoxynivalenol (DON) in 55 wheat food and feed samples, 26 from conventional and 29 from organic production. Immunoaffinity columns prepared by entrapping anti-DON antibodies by the sol-gel method were used for sample clean-up. DON was quantified by high performance liquid chromatography (HPLC) and ultraviolet (UV) detection. In general, the incidence of DON contamination was rather low. In eight samples (14.5%) the DON concentration was above the LOQ (380 ng/g), in six samples (10.9%) DON was detected but could not be quantified (>LOD (200 ng/g), LOQ. The data indicate both a higher incidence of DON contamination and higher DON concentrations in food and feed samples from conventional than in those from organic production.

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DO - 10.1016/j.jchromb.2009.08.016

M3 - Article

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VL - 878

SP - 187

EP - 193

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 2

ER -

Determination of deoxynivalenol in organic and conventional food and feed by sol-gel immunoaffinity chromatography and HPLC-UV detection

Abstract

Austrian Fields of Science 2012

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