TY - JOUR
T1 - Determination of heterocyclic aromatic amines in beef extract, cooked meat and rat urine by liquid chromatography with coulometric electrode array detection
AU - Gerbl, U
AU - Cichna-Markl, Margit
AU - Zsivkovits, Markus
AU - Knasmüller, Siegfried
AU - Sontag, Gerhard
N1 - DOI: 10.1016/j.jchromb.2003.10.063
Coden: JCBAA
Affiliations: Institute for Analytical Chemistry, University of Vienna, Waehringer Straße 38, Vienna A-1090, Austria; Institute of Cancer Research, Borschkegasse 8a, Vienna A-1090, Austria
Adressen: Sontag, G.; Institute for Analytical Chemistry; University of Vienna; Waehringer Straße 38 Vienna A-1090, Austria; email: [email protected]
Source-File: AnalytChemScopus.csv
Import aus Scopus: 2-s2.0-1242351293
Importdatum: 12.02.2007 12:03:03
22.10.2007: Datenanforderung 1934 (Import Sachbearbeiter)
PY - 2004
Y1 - 2004
N2 - This paper describes a method for the determination of heterocyclic aromatic amines (HAs; DMIP, IQ, MeIQ, MeIQx, 4,8-DiMeIQx, 7,8-DiMeIQx, AaC, PhIP) by high-performance liquid chromatography (HPLC) with coulometric electrode array detection. The compounds are separated on reversed phase columns (LiChroCart Superspher 60 RP-select B, 250mm×2mm, 4œm and LiChrospher 60 RP-select B, 250mm×4mm, 5œm) using mobile phases consisting of acetonitrile/buffer/distilled water and detected at eight working electrodes at potentials between +190 and +680mV against modified palladium electrodes. In the context of an EU-interlaboratory exercise, the method was applied to analyse a standardised test solution and - after isolation of the analytes by several clean-up steps - for the analysis of standardised beef extract and grilled meat. Further, the method could be applied for the analysis of HAs in suspensions of bacteria and rat urine without any sample preparation step beyond sample dilution. The data obtained show that HPLC with coulometric electrode array detection gives accurate results. Œ 2003 Elsevier B.V. All rights reserved.
AB - This paper describes a method for the determination of heterocyclic aromatic amines (HAs; DMIP, IQ, MeIQ, MeIQx, 4,8-DiMeIQx, 7,8-DiMeIQx, AaC, PhIP) by high-performance liquid chromatography (HPLC) with coulometric electrode array detection. The compounds are separated on reversed phase columns (LiChroCart Superspher 60 RP-select B, 250mm×2mm, 4œm and LiChrospher 60 RP-select B, 250mm×4mm, 5œm) using mobile phases consisting of acetonitrile/buffer/distilled water and detected at eight working electrodes at potentials between +190 and +680mV against modified palladium electrodes. In the context of an EU-interlaboratory exercise, the method was applied to analyse a standardised test solution and - after isolation of the analytes by several clean-up steps - for the analysis of standardised beef extract and grilled meat. Further, the method could be applied for the analysis of HAs in suspensions of bacteria and rat urine without any sample preparation step beyond sample dilution. The data obtained show that HPLC with coulometric electrode array detection gives accurate results. Œ 2003 Elsevier B.V. All rights reserved.
U2 - 10.1016/j.jchromb.2003.10.063
DO - 10.1016/j.jchromb.2003.10.063
M3 - Article
SN - 1570-0232
VL - 802
SP - 107
EP - 113
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1
ER -