Abstract
The paper presents a novel real-time PCR method allowing the detection of traces of celery (Apium graveolens) in complex food matrices. The method is based on the amplification of a sequence of the gene coding for the Apium graveolens NADPH-dependent mannose-6-phosphate reductase. It allows the detection of three commonly used celery varieties, celery roots (Apium graveolens var. rapaceum), celery stalks (Apium graveolens var. dulce) and leaf celery (Apium graveolens var. secalinum) and does not show any cross-reactivity with 64 biological species, including ten members of the Apiaceae family. The limit of detection, determined by analysing serially diluted celery extracts, is 10 pg celery DNA for all three celery varieties. In spiked model sausages, the LOD is 0.005% celery. The real-time PCR method was applied to 26 commercial food products. Celery DNA was found in one out of ten samples without any information about the presence of celery. © 2011 Elsevier Ltd. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 189-195 |
| Number of pages | 7 |
| Journal | Food Chemistry |
| Volume | 130 |
| Issue number | 1 |
| Publication status | Published - 2012 |
Austrian Fields of Science 2012
- 104002 Analytical chemistry
- 104009 Food chemistry