Engineering Terpene Biosynthesis in Streptomyces for Production of the Advanced Biofuel Precursor Bisabolene

Ryan M. Phelan, Olga N. Sekurova, Jay D. Keasling, Sergey B. Zotchev

    Publications: Contribution to journalArticlePeer Reviewed

    Abstract

    The past decade has witnessed a large influx of research toward the creation of sustainable, biologically derived fuels. While significant effort has been exerted to improve production capacity in common hosts, such as Escherichia coli or Saccharomyces cerevisiae, studies concerning alternate microbes comparatively lag. In an effort to expand the breadth of characterized hosts for fuel production, we map the terpene biosynthetic pathway in a model actinobacterium, Streptomyces venezuelae, and further alter secondary metabolism to afford the advanced biofuel precursor bisabolene. Leveraging information gained from study of the native isoprenoid pathway, we were able to increase bisabolene titer nearly 5-fold over the base production strain, more than 2 orders of magnitude greater than the combined terpene yield in the wild-type host. We also explored production on carbon sources of varying complexity to, notably, define this host as one able to perform consolidated bioprocessing.

    Original languageEnglish
    Pages (from-to)393-399
    Number of pages7
    JournalACS Synthetic Biology
    Volume4
    Issue number4
    DOIs
    Publication statusPublished - Apr 2015

    Austrian Fields of Science 2012

    • 106013 Genetics
    • 106022 Microbiology

    Keywords

    • Streptomyces
    • isoprenoid
    • terpene
    • biofuel
    • consolidated bioprocessing
    • NOURSEI ATCC 11455
    • MICROBIAL-PRODUCTION
    • ESCHERICHIA-COLI
    • GENE-CLUSTER
    • IDENTIFICATION
    • COELICOLOR
    • NYSTATIN
    • GEOSMIN
    • PATHWAY
    • BIOMASS

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