Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid

Abhinav Kumar; Elif Melis Bicer; Anna Babin Morgan; Paul E. Pfeffer; Marco Monopoli; Kenneth A. Dawson; Jonny Eriksson; Katarina Edwards; Steven Lynham; Matthew Arno; Annelie F. Behndig; Anders Blomberg; Graham Somers; Dave Hassall; Lea Ann Dailey; Ben Forbes; Ian S. Mudway

doi:10.1016/j.nano.2015.12.369

Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid

Abhinav Kumar (Corresponding author)
, Elif Melis Bicer
, Anna Babin Morgan
, Paul E. Pfeffer
, Marco Monopoli
, Kenneth A. Dawson
, Jonny Eriksson
, Katarina Edwards
, Steven Lynham
, Matthew Arno
, Annelie F. Behndig
, Anders Blomberg
, Graham Somers
, Dave Hassall
, Lea Ann Dailey
, Ben Forbes
, Ian S. Mudway

Publications: Contribution to journal › Article › Peer Reviewed

Abstract

When inhaled nanoparticles deposit in the lungs, they transit through respiratory tract lining fluid (RTLF) acquiring a biomolecular corona reflecting the interaction of the RTLF with the nanomaterial surface. Label-free snapshot proteomics was used to generate semi-quantitative profiles of corona proteins formed around silica (SiO₂) and poly(vinyl) acetate (PVAc) nanoparticles in RTLF, the latter employed as an archetype drug delivery vehicle. The evolved PVAc corona was significantly enriched compared to that observed on SiO₂ nanoparticles (698 vs. 429 proteins identified); however both coronas contained a substantial contribution from innate immunity proteins, including surfactant protein A, napsin A and complement (C1q and C3) proteins. Functional protein classification supports the hypothesis that corona formation in RTLF constitutes opsonisation, preparing particles for phagocytosis and clearance from the lungs. These data highlight how an understanding of the evolved corona is necessary for the design of inhaled nanomedicines with acceptable safety and tailored clearance profiles.

Original language	English
Pages (from-to)	1033-1043
Number of pages	11
Journal	Nanomedicine: Nanotechnology, Biology, and Medicine
Volume	12
Issue number	4
Early online date	6 Jan 2016
DOIs	https://doi.org/10.1016/j.nano.2015.12.369
Publication status	Published - May 2016
Externally published	Yes

Funding

Funding: The corona work was funded under the QualityNano (EU-FP7) scheme, and by the Swedish Heart-Lung Foundation and Västerbotten County Council, Sweden. Elif Melis Bicer was supported by a BBSRC-CASE studentship, in association with GlaxoSmithKline Research & Development Ltd.

Austrian Fields of Science 2012

301211 Toxicology

Keywords

Nanoparticle
Plasma
Poly(vinyl) acetate
Protein corona
Proteomics
Respiratory tract lining fluid
Silica

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10.1016/j.nano.2015.12.369

Cite this

Kumar, A., Bicer, E. M., Morgan, A. B., Pfeffer, P. E., Monopoli, M., Dawson, K. A., Eriksson, J., Edwards, K., Lynham, S., Arno, M., Behndig, A. F., Blomberg, A., Somers, G., Hassall, D., Dailey, L. A., Forbes, B., & Mudway, I. S. (2016). Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid. Nanomedicine: Nanotechnology, Biology, and Medicine, 12(4), 1033-1043. https://doi.org/10.1016/j.nano.2015.12.369

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title = "Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid",

abstract = "When inhaled nanoparticles deposit in the lungs, they transit through respiratory tract lining fluid (RTLF) acquiring a biomolecular corona reflecting the interaction of the RTLF with the nanomaterial surface. Label-free snapshot proteomics was used to generate semi-quantitative profiles of corona proteins formed around silica (SiO2) and poly(vinyl) acetate (PVAc) nanoparticles in RTLF, the latter employed as an archetype drug delivery vehicle. The evolved PVAc corona was significantly enriched compared to that observed on SiO2 nanoparticles (698 vs. 429 proteins identified); however both coronas contained a substantial contribution from innate immunity proteins, including surfactant protein A, napsin A and complement (C1q and C3) proteins. Functional protein classification supports the hypothesis that corona formation in RTLF constitutes opsonisation, preparing particles for phagocytosis and clearance from the lungs. These data highlight how an understanding of the evolved corona is necessary for the design of inhaled nanomedicines with acceptable safety and tailored clearance profiles.",

keywords = "Nanoparticle, Plasma, Poly(vinyl) acetate, Protein corona, Proteomics, Respiratory tract lining fluid, Silica",

author = "Abhinav Kumar and Bicer, \{Elif Melis\} and Morgan, \{Anna Babin\} and Pfeffer, \{Paul E.\} and Marco Monopoli and Dawson, \{Kenneth A.\} and Jonny Eriksson and Katarina Edwards and Steven Lynham and Matthew Arno and Behndig, \{Annelie F.\} and Anders Blomberg and Graham Somers and Dave Hassall and Dailey, \{Lea Ann\} and Ben Forbes and Mudway, \{Ian S.\}",

note = "Funding Information: Funding: The corona work was funded under the QualityNano (EU-FP7) scheme, and by the Swedish Heart-Lung Foundation and V{\"a}sterbotten County Council, Sweden. Elif Melis Bicer was supported by a BBSRC-CASE studentship, in association with GlaxoSmithKline Research \& Development Ltd. Publisher Copyright: {\textcopyright} 2015.",

year = "2016",

month = may,

doi = "10.1016/j.nano.2015.12.369",

language = "English",

volume = "12",

pages = "1033--1043",

journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",

issn = "1549-9634",

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Kumar, A, Bicer, EM, Morgan, AB, Pfeffer, PE, Monopoli, M, Dawson, KA, Eriksson, J, Edwards, K, Lynham, S, Arno, M, Behndig, AF, Blomberg, A, Somers, G, Hassall, D, Dailey, LA, Forbes, B & Mudway, IS 2016, 'Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid', Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 12, no. 4, pp. 1033-1043. https://doi.org/10.1016/j.nano.2015.12.369

Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid. / Kumar, Abhinav (Corresponding author); Bicer, Elif Melis; Morgan, Anna Babin et al.
In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 12, No. 4, 05.2016, p. 1033-1043.

Publications: Contribution to journal › Article › Peer Reviewed

TY - JOUR

T1 - Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid

AU - Kumar, Abhinav

AU - Bicer, Elif Melis

AU - Morgan, Anna Babin

AU - Pfeffer, Paul E.

AU - Monopoli, Marco

AU - Dawson, Kenneth A.

AU - Eriksson, Jonny

AU - Edwards, Katarina

AU - Lynham, Steven

AU - Arno, Matthew

AU - Behndig, Annelie F.

AU - Blomberg, Anders

AU - Somers, Graham

AU - Hassall, Dave

AU - Dailey, Lea Ann

AU - Forbes, Ben

AU - Mudway, Ian S.

N1 - Funding Information: Funding: The corona work was funded under the QualityNano (EU-FP7) scheme, and by the Swedish Heart-Lung Foundation and Västerbotten County Council, Sweden. Elif Melis Bicer was supported by a BBSRC-CASE studentship, in association with GlaxoSmithKline Research & Development Ltd. Publisher Copyright: © 2015.

PY - 2016/5

Y1 - 2016/5

N2 - When inhaled nanoparticles deposit in the lungs, they transit through respiratory tract lining fluid (RTLF) acquiring a biomolecular corona reflecting the interaction of the RTLF with the nanomaterial surface. Label-free snapshot proteomics was used to generate semi-quantitative profiles of corona proteins formed around silica (SiO2) and poly(vinyl) acetate (PVAc) nanoparticles in RTLF, the latter employed as an archetype drug delivery vehicle. The evolved PVAc corona was significantly enriched compared to that observed on SiO2 nanoparticles (698 vs. 429 proteins identified); however both coronas contained a substantial contribution from innate immunity proteins, including surfactant protein A, napsin A and complement (C1q and C3) proteins. Functional protein classification supports the hypothesis that corona formation in RTLF constitutes opsonisation, preparing particles for phagocytosis and clearance from the lungs. These data highlight how an understanding of the evolved corona is necessary for the design of inhaled nanomedicines with acceptable safety and tailored clearance profiles.

AB - When inhaled nanoparticles deposit in the lungs, they transit through respiratory tract lining fluid (RTLF) acquiring a biomolecular corona reflecting the interaction of the RTLF with the nanomaterial surface. Label-free snapshot proteomics was used to generate semi-quantitative profiles of corona proteins formed around silica (SiO2) and poly(vinyl) acetate (PVAc) nanoparticles in RTLF, the latter employed as an archetype drug delivery vehicle. The evolved PVAc corona was significantly enriched compared to that observed on SiO2 nanoparticles (698 vs. 429 proteins identified); however both coronas contained a substantial contribution from innate immunity proteins, including surfactant protein A, napsin A and complement (C1q and C3) proteins. Functional protein classification supports the hypothesis that corona formation in RTLF constitutes opsonisation, preparing particles for phagocytosis and clearance from the lungs. These data highlight how an understanding of the evolved corona is necessary for the design of inhaled nanomedicines with acceptable safety and tailored clearance profiles.

KW - Nanoparticle

KW - Plasma

KW - Poly(vinyl) acetate

KW - Protein corona

KW - Proteomics

KW - Respiratory tract lining fluid

KW - Silica

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DO - 10.1016/j.nano.2015.12.369

M3 - Article

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AN - SCOPUS:84961216142

SN - 1549-9634

VL - 12

SP - 1033

EP - 1043

JO - Nanomedicine: Nanotechnology, Biology, and Medicine

JF - Nanomedicine: Nanotechnology, Biology, and Medicine

IS - 4

ER -

Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid

Abstract

Funding

Austrian Fields of Science 2012

Keywords

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