Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity

Dana E Hunt; Vanja Klepac-Ceraj; Silvia G Acinas; Clement Gautier; Stefan Bertilsson; Martin F Polz

doi:10.1128/AEM.72.3.2221-2225.2006

Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity

Dana E Hunt, Vanja Klepac-Ceraj, Silvia G Acinas, Clement Gautier, Stefan Bertilsson, Martin F Polz

Publications: Contribution to journal › Article › Peer Reviewed

Abstract

The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample.

Original language	English
Pages (from-to)	2221-5
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	72
Issue number	3
DOIs	https://doi.org/10.1128/AEM.72.3.2221-2225.2006
Publication status	Published - Mar 2006

Austrian Fields of Science 2012

106022 Microbiology

Keywords

Bacteria/classification
Base Sequence
DNA Primers
Gene Library
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction/methods
RNA, Ribosomal, 23S/genetics
Sequence Analysis, DNA

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10.1128/AEM.72.3.2221-2225.2006

Cite this

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abstract = "The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample.",

keywords = "Bacteria/classification, Base Sequence, DNA Primers, Gene Library, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction/methods, RNA, Ribosomal, 23S/genetics, Sequence Analysis, DNA",

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AU - Klepac-Ceraj, Vanja

AU - Acinas, Silvia G

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AU - Bertilsson, Stefan

AU - Polz, Martin F

PY - 2006/3

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AB - The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample.

KW - Bacteria/classification

KW - Base Sequence

KW - DNA Primers

KW - Gene Library

KW - Molecular Sequence Data

KW - Phylogeny

KW - Polymerase Chain Reaction/methods

KW - RNA, Ribosomal, 23S/genetics

KW - Sequence Analysis, DNA

U2 - 10.1128/AEM.72.3.2221-2225.2006

DO - 10.1128/AEM.72.3.2221-2225.2006

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SP - 2221

EP - 2225

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 3

ER -

Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity

Abstract

Austrian Fields of Science 2012

Keywords

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Cite this