Evidence for novel tomato seed allergens: Ige-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling

Olivia Y. Bässler; Julia Weiss; Stefanie Wienkoop; Karola Lehmann; Christian Scheler; Sabine Dölle; Dietmar Schwarz; Philipp Franken; Eckhard George; Margitta Worm; Wolfram Weckwerth

doi:10.1021/pr800186d

Evidence for novel tomato seed allergens: Ige-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling

Olivia Y. Bässler, Julia Weiss, Stefanie Wienkoop, Karola Lehmann, Christian Scheler, Sabine Dölle, Dietmar Schwarz, Philipp Franken, Eckhard George, Margitta Worm, Wolfram Weckwerth (Corresponding author)

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Abstract

Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomatosensitized patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin (Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher than tomato fruit protein. For the molecular characterization of tomato seed allergens, a multidimensional protein fractionation strategy and LC-MS/MS was used. Two legumin- and vicilin-proteins were purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying IgE-sensitivity against the purified proteins. In silico structural modeling indicates high homology between epitopes of known walnut allergens and the detected IgE-crossreactive tomato proteins

Original language	English
Pages (from-to)	1111-1122
Number of pages	12
Journal	Journal of Proteome Research
Volume	8
Issue number	3
DOIs	https://doi.org/10.1021/pr800186d
Publication status	Published - 2009

Austrian Fields of Science 2012

106002 Biochemistry
1030 Physics, Astronomy
106037 Proteomics

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Bässler, O. Y., Weiss, J., Wienkoop, S., Lehmann, K., Scheler, C., Dölle, S., Schwarz, D., Franken, P., George, E., Worm, M., & Weckwerth, W. (2009). Evidence for novel tomato seed allergens: Ige-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling. Journal of Proteome Research, 8(3), 1111-1122. https://doi.org/10.1021/pr800186d

@article{dc4289a33f374de09503dcf06fac104a,

title = "Evidence for novel tomato seed allergens: Ige-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling",

abstract = "Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomatosensitized patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin (Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher than tomato fruit protein. For the molecular characterization of tomato seed allergens, a multidimensional protein fractionation strategy and LC-MS/MS was used. Two legumin- and vicilin-proteins were purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying IgE-sensitivity against the purified proteins. In silico structural modeling indicates high homology between epitopes of known walnut allergens and the detected IgE-crossreactive tomato proteins",

author = "B{\"a}ssler, {Olivia Y.} and Julia Weiss and Stefanie Wienkoop and Karola Lehmann and Christian Scheler and Sabine D{\"o}lle and Dietmar Schwarz and Philipp Franken and Eckhard George and Margitta Worm and Wolfram Weckwerth",

year = "2009",

doi = "10.1021/pr800186d",

language = "English",

volume = "8",

pages = "1111--1122",

journal = "Journal of Proteome Research",

issn = "1535-3893",

publisher = "AMER CHEMICAL SOC",

number = "3",

}

Bässler, OY, Weiss, J, Wienkoop, S, Lehmann, K, Scheler, C, Dölle, S, Schwarz, D, Franken, P, George, E, Worm, M & Weckwerth, W 2009, 'Evidence for novel tomato seed allergens: Ige-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling', Journal of Proteome Research, vol. 8, no. 3, pp. 1111-1122. https://doi.org/10.1021/pr800186d

Evidence for novel tomato seed allergens: Ige-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling. / Bässler, Olivia Y.; Weiss, Julia; Wienkoop, Stefanie et al.
In: Journal of Proteome Research, Vol. 8, No. 3, 2009, p. 1111-1122.

Publications: Contribution to journal › Article › Peer Reviewed

TY - JOUR

T1 - Evidence for novel tomato seed allergens: Ige-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling

AU - Bässler, Olivia Y.

AU - Weiss, Julia

AU - Wienkoop, Stefanie

AU - Lehmann, Karola

AU - Scheler, Christian

AU - Dölle, Sabine

AU - Schwarz, Dietmar

AU - Franken, Philipp

AU - George, Eckhard

AU - Worm, Margitta

AU - Weckwerth, Wolfram

PY - 2009

Y1 - 2009

N2 - Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomatosensitized patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin (Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher than tomato fruit protein. For the molecular characterization of tomato seed allergens, a multidimensional protein fractionation strategy and LC-MS/MS was used. Two legumin- and vicilin-proteins were purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying IgE-sensitivity against the purified proteins. In silico structural modeling indicates high homology between epitopes of known walnut allergens and the detected IgE-crossreactive tomato proteins

AB - Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomatosensitized patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin (Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher than tomato fruit protein. For the molecular characterization of tomato seed allergens, a multidimensional protein fractionation strategy and LC-MS/MS was used. Two legumin- and vicilin-proteins were purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying IgE-sensitivity against the purified proteins. In silico structural modeling indicates high homology between epitopes of known walnut allergens and the detected IgE-crossreactive tomato proteins

U2 - 10.1021/pr800186d

DO - 10.1021/pr800186d

M3 - Article

SN - 1535-3893

VL - 8

SP - 1111

EP - 1122

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 3

ER -

Evidence for novel tomato seed allergens: Ige-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling

Abstract

Austrian Fields of Science 2012

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