TY - JOUR
T1 - Ex vivo instability of lipids in whole blood: preanalytical recommendations for clinical lipidomics studies
AU - Wang, Qingqing
AU - Hoene, Miriam
AU - Hu, Chunxiu
AU - Fritsche, Louise
AU - Ahrends, Robert
AU - Liebisch, Gerhard
AU - Ekroos, Kim
AU - Fritsche, Andreas
AU - Birkenfeld, Andreas L.
AU - Liu, Xinyu
AU - Zhao, Xinjie
AU - Li, Qi
AU - Su, Benzhe
AU - Peter, Andreas
AU - Xu, Guowang
AU - Lehmann, Rainer
N1 - Accession Number: WOS:001001814300001
PubMed ID: 37087100
PY - 2023/6
Y1 - 2023/6
N2 - Reliability, robustness, and interlaboratory comparability of quantitative measurements is critical for clinical lipidomics studies. Lipids’ different ex vivo stability in blood bears the risk of misinterpretation of data. Clear recommendations for the process of blood sample collection are required. We studied by UHPLC-high resolution mass spectrometry, as part of the “Preanalytics interest group” of the International Lipidomics Society, the stability of 417 lipid species in EDTA whole blood after exposure to either 4◦C, 21◦C, or 30◦C at six different time points (0.5 h–24 h) to cover common daily routine conditions in clinical settings. In total, >800 samples were analyzed. 325 and 288 robust lipid species resisted 24 h exposure of EDTA whole blood to 21◦C or 30◦C, respectively. Most significant instabilities were detected for FA, LPE, and LPC. Based on our data, we recommend cooling whole blood at once and permanent. Plasma should be separated within 4 h, unless the focus is solely on robust lipids. Lists are provided to check the ex vivo (in)stability of distinct lipids and potential biomarkers of interest in whole blood. To conclude, our results contribute to the international efforts towards reliable and comparable clinical lipidomics data paving the way to the proper diagnostic application of distinct lipid patterns or lipid profiles in the future.
AB - Reliability, robustness, and interlaboratory comparability of quantitative measurements is critical for clinical lipidomics studies. Lipids’ different ex vivo stability in blood bears the risk of misinterpretation of data. Clear recommendations for the process of blood sample collection are required. We studied by UHPLC-high resolution mass spectrometry, as part of the “Preanalytics interest group” of the International Lipidomics Society, the stability of 417 lipid species in EDTA whole blood after exposure to either 4◦C, 21◦C, or 30◦C at six different time points (0.5 h–24 h) to cover common daily routine conditions in clinical settings. In total, >800 samples were analyzed. 325 and 288 robust lipid species resisted 24 h exposure of EDTA whole blood to 21◦C or 30◦C, respectively. Most significant instabilities were detected for FA, LPE, and LPC. Based on our data, we recommend cooling whole blood at once and permanent. Plasma should be separated within 4 h, unless the focus is solely on robust lipids. Lists are provided to check the ex vivo (in)stability of distinct lipids and potential biomarkers of interest in whole blood. To conclude, our results contribute to the international efforts towards reliable and comparable clinical lipidomics data paving the way to the proper diagnostic application of distinct lipid patterns or lipid profiles in the future.
KW - blood
KW - Clinical lipidomics
KW - lipid stability
KW - preanalytical
KW - sample collection
UR - http://www.scopus.com/inward/record.url?scp=85160316552&partnerID=8YFLogxK
U2 - 10.1016/j.jlr.2023.100378
DO - 10.1016/j.jlr.2023.100378
M3 - Article
C2 - 37087100
AN - SCOPUS:85160316552
SN - 0022-2275
VL - 64
JO - Journal of Lipid Research
JF - Journal of Lipid Research
IS - 6
M1 - 100378
ER -