Expression, Purification and Crystallization of Wheat Profilin (Tri a 12)

Christina Ecker; Peter Forstenlechner; Dalibor Milic; Karin Hoffmann-Sommergruber; Margit Cichna-Markl; Dubravka Matkovic-Calogovic

Expression, Purification and Crystallization of Wheat Profilin (Tri a 12)

Christina Ecker, Peter Forstenlechner, Dalibor Milic, Karin Hoffmann-Sommergruber, Margit Cichna-Markl, Dubravka Matkovic-Calogovic (Corresponding author)

Department of Analytical Chemistry

Publications: Contribution to journal › Article › Peer Reviewed

Abstract

Profilin from wheat (Triticum aestivum) has been identified as an allergen (Tri a 12). The recombinant 14 kDa protein was produced in Escherichia coil, purified and crystallized using the hanging-drop vapour-diffusion method. A diffraction-data set was collected in-house from a single crystal to a resolution of 3.3 angstrom. The crystals belonged to space group P3(2)21, with unit-cell parameters a = b = 58.9 angstrom, c = 82.5 angstrom, alpha= beta = 90 degrees and gamma = 120 degrees. (doi: 10.5562/cca1790)

Original language	English
Pages (from-to)	419-422
Number of pages	4
Journal	Croatica Chemica Acta
Volume	84
Issue number	3
Publication status	Published - 2011

Austrian Fields of Science 2012

104002 Analytical chemistry
104009 Food chemistry

Cite this

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abstract = "Profilin from wheat (Triticum aestivum) has been identified as an allergen (Tri a 12). The recombinant 14 kDa protein was produced in Escherichia coil, purified and crystallized using the hanging-drop vapour-diffusion method. A diffraction-data set was collected in-house from a single crystal to a resolution of 3.3 angstrom. The crystals belonged to space group P3(2)21, with unit-cell parameters a = b = 58.9 angstrom, c = 82.5 angstrom, alpha= beta = 90 degrees and gamma = 120 degrees. (doi: 10.5562/cca1790)",

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AU - Ecker, Christina

AU - Forstenlechner, Peter

AU - Milic, Dalibor

AU - Hoffmann-Sommergruber, Karin

AU - Cichna-Markl, Margit

AU - Matkovic-Calogovic, Dubravka

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PY - 2011

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N2 - Profilin from wheat (Triticum aestivum) has been identified as an allergen (Tri a 12). The recombinant 14 kDa protein was produced in Escherichia coil, purified and crystallized using the hanging-drop vapour-diffusion method. A diffraction-data set was collected in-house from a single crystal to a resolution of 3.3 angstrom. The crystals belonged to space group P3(2)21, with unit-cell parameters a = b = 58.9 angstrom, c = 82.5 angstrom, alpha= beta = 90 degrees and gamma = 120 degrees. (doi: 10.5562/cca1790)

AB - Profilin from wheat (Triticum aestivum) has been identified as an allergen (Tri a 12). The recombinant 14 kDa protein was produced in Escherichia coil, purified and crystallized using the hanging-drop vapour-diffusion method. A diffraction-data set was collected in-house from a single crystal to a resolution of 3.3 angstrom. The crystals belonged to space group P3(2)21, with unit-cell parameters a = b = 58.9 angstrom, c = 82.5 angstrom, alpha= beta = 90 degrees and gamma = 120 degrees. (doi: 10.5562/cca1790)

M3 - Article

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VL - 84

SP - 419

EP - 422

JO - Croatica Chemica Acta

JF - Croatica Chemica Acta

IS - 3

ER -

Expression, Purification and Crystallization of Wheat Profilin (Tri a 12)

Abstract

Austrian Fields of Science 2012

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