TY - JOUR
T1 - Eye proteome of Drosophila melanogaster
AU - Kumar, Mukesh
AU - Has, Canan
AU - Lam-Kamath, Khanh
AU - Ayciriex, Sophie
AU - Dewett, Deepshe
AU - Bashir, Mhamed
AU - Poupault, Clara
AU - Schuhmann, Kai
AU - Thomas, Henrik
AU - Knittelfelder, Oskar
AU - Raghuraman, Bharath Kumar
AU - Ahrends, Robert
AU - Rister, Jens
AU - Shevchenko, Andrej
N1 - Accession Number: WOS:001123561200001
PubMed ID: 37963819
PY - 2024/5
Y1 - 2024/5
N2 - Drosophila melanogaster is a popular model organism to elucidate the molecular mechanisms that underlie the structure and function of the eye as well as the causes of retinopathies, aging, light-induced damage, or dietary deficiencies. Large-scale screens have isolated genes whose mutation causes morphological and functional ocular defects, which led to the discovery of key components of the phototransduction cascade. However, the proteome of the Drosophila eye is poorly characterized. Here, we used GeLC-MS/MS to quantify 3516 proteins, including the absolute (molar) quantities of 43 proteins in the eye of adult male Drosophila reared on standard laboratory food. This work provides a generic and expandable resource for further genetic, pharmacological, and dietary studies.
AB - Drosophila melanogaster is a popular model organism to elucidate the molecular mechanisms that underlie the structure and function of the eye as well as the causes of retinopathies, aging, light-induced damage, or dietary deficiencies. Large-scale screens have isolated genes whose mutation causes morphological and functional ocular defects, which led to the discovery of key components of the phototransduction cascade. However, the proteome of the Drosophila eye is poorly characterized. Here, we used GeLC-MS/MS to quantify 3516 proteins, including the absolute (molar) quantities of 43 proteins in the eye of adult male Drosophila reared on standard laboratory food. This work provides a generic and expandable resource for further genetic, pharmacological, and dietary studies.
KW - absolute quantification
KW - Drosophila melanogaster
KW - eye proteome
KW - phototransduction
UR - http://www.scopus.com/inward/record.url?scp=85176929865&partnerID=8YFLogxK
U2 - 10.1002/pmic.202300330
DO - 10.1002/pmic.202300330
M3 - Article
AN - SCOPUS:85176929865
SN - 1615-9853
VL - 24
JO - Proteomics
JF - Proteomics
IS - 10
M1 - 2300330
ER -