@article{3c13f52e2304469fb389d86ee98ab348,
title = "Functional vertical connectivity of microbial communities in the ocean",
abstract = "Sinking particles are a critical conduit for the transport of surface microbes to the ocean's interior. Vertical connectivity of phylogenetic composition has been shown; however, the functional vertical connectivity of microbial communities has not yet been explored in detail. We investigated protein and taxa profiles of both free-living and particle-attached microbial communities from the surface to 3000 m depth using a combined metaproteomic and 16 S rRNA amplicon sequencing approach. A clear compositional and functional vertical connectivity of microbial communities was observed throughout the water column with Oceanospirillales, Alteromonadales, and Rhodobacterales as key taxa. The surface-derived particle-associated microbes increased the expression of proteins involved in basic metabolism, organic matter processing, and environmental stress response in deep waters. This study highlights the functional vertical connectivity between surface and deep-sea microbial communities via sinking particles and reveals that a considerable proportion of the deep-sea microbes might originate from surface waters and have a major impact on the biogeochemical cycles in the deep sea. ",
keywords = "Oceans and Seas, RNA, Ribosomal, 16S/genetics, Microbiota, Seawater/microbiology, Phylogeny, Bacteria/genetics",
author = "Shi Chen and Zhang-Xian Xie and Ke-Qiang Yan and Jian-Wei Chen and Dong-Xu Li and Peng-Fei Wu and Ling Peng and Lin Lin and Chun-Ming Dong and Zihao Zhao and Guang-Yi Fan and Si-Qi Liu and Herndl, {Gerhard J} and Da-Zhi Wang",
note = "Funding Information: Acknowledgments: We thank the crew of Rv tAn KAh Kee for the help during the research cruise. We thank h. lin and Z. hu in Xiamen University for providing the hydrography plots in fig. S1. We also thank the reviewers for the constructive criticism that has helped us to improve the overall merit of the manuscript. Funding: d.-Z.W. was supported by the national Key Research and development Program of China (no. 2022YFC3105302) and the national natural Science Foundation of China (no. 41425021). G.J.h. was supported by the Austrian Science Fund (FWF) grant P 35587-B. G.J.h. and Z.-X.X. were both supported by a visiting Fellowship from State Key laboratory of Marine environmental Science (Xiamen University). Author contributions: d.-Z.W. and Z.-X.X. proposed the idea for this study. d.-X.l. and P.-F.W. helped with sample collection. S.C., Z.-X.X., K.-Q.Y., J.-W.C., l.l., l.P., C.-M.d., G.-Y.F. and S.Q.l. conducted the analysis. S.C. drafted the manuscript. G.J.h., d.-Z.W., Z.-X.X. and Z.Z. helped with revising the manuscript. G.J.h., Z.-X.X., and d.-Z.W. finalized the manuscript. Competing interests: the authors declare that they have no competing interests. Data and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. Raw reads of 16S rRnA gene amplicon sequencing data have been deposited in the nCBi database under the BioProject accession number PRJnA989431. Raw data of metaproteomics can be found in the ProteomeXchange Consortium via the PRide Partner Repository under dataset identifiers PXd018513 and PXd026838. Publisher Copyright: {\textcopyright} 2024 The Authors.",
year = "2024",
month = may,
day = "24",
doi = "10.1126/sciadv.adj8184",
language = "English",
volume = "10",
journal = "Science Advances",
issn = "2375-2548",
publisher = "AMER ASSOC ADVANCEMENT SCIENCE",
number = "21",
}
