TY - JOUR
T1 - Genome Mining of Streptomyces sp. YIM 130001 Isolated From Lichen Affords New Thiopeptide Antibiotic
AU - Schneider, Olha
AU - Simic, Nebojsa
AU - Aachmann, Finn Lillelund
AU - Rueckert, Christian
AU - Kristiansen, Kare Andre
AU - Kalinowski, Joern
AU - Jiang, Yi
AU - Wang, Lisong
AU - Jiang, Cheng-Lin
AU - Lale, Rahmi
AU - Zotchev, Sergey B.
N1 - Publisher Copyright:
© Copyright © 2018 Schneider, Simic, Aachmann, Rückert, Kristiansen, Kalinowski, Jiang, Wang, Jiang, Lale and Zotchev.
PY - 2018/12/19
Y1 - 2018/12/19
N2 - Streptomyces bacteria are recognized as an important source for antibiotics with broad applications in human medicine and animal health. Here, we report the isolation of a new lichen-associating Streptomyces sp. YIM 130001 from the tropical rainforest in Xishuangbanna (Yunnan, China), which displayed antibacterial activity against Bacillus subtilis. The draft genome sequence of this isolate strain revealed 18 putative biosynthetic gene clusters (BGCs) for secondary metabolites, which is an unusually low number compared to a typical streptomycete. Inactivation of a lantibiotic dehydrogenase-encoding gene from the BGC presumed to govern biosynthesis of a thiopeptide resulted in the loss of bioactivity. Using comparative HPLC analysis, two peaks in the chromatogram were identified in the extract from the wild-type strain, which were missing in the extract from the mutant. The compounds corresponding to the identified peaks were purified, and structure of one compound was elucidated using NMR. The compound, designated geninthiocin B, showed high similarity to several 35-membered macrocyclic thiopeptides geninthiocin, Val-geninthiocin and berninamycin A. Bioinformatics analysis of the geninthiocin B BGC revealed its close homology to that of berninamycins.
AB - Streptomyces bacteria are recognized as an important source for antibiotics with broad applications in human medicine and animal health. Here, we report the isolation of a new lichen-associating Streptomyces sp. YIM 130001 from the tropical rainforest in Xishuangbanna (Yunnan, China), which displayed antibacterial activity against Bacillus subtilis. The draft genome sequence of this isolate strain revealed 18 putative biosynthetic gene clusters (BGCs) for secondary metabolites, which is an unusually low number compared to a typical streptomycete. Inactivation of a lantibiotic dehydrogenase-encoding gene from the BGC presumed to govern biosynthesis of a thiopeptide resulted in the loss of bioactivity. Using comparative HPLC analysis, two peaks in the chromatogram were identified in the extract from the wild-type strain, which were missing in the extract from the mutant. The compounds corresponding to the identified peaks were purified, and structure of one compound was elucidated using NMR. The compound, designated geninthiocin B, showed high similarity to several 35-membered macrocyclic thiopeptides geninthiocin, Val-geninthiocin and berninamycin A. Bioinformatics analysis of the geninthiocin B BGC revealed its close homology to that of berninamycins.
KW - new Streptomyces sp. from lichen
KW - antibacterial activity
KW - genome mining
KW - new thiopeptide antibiotic
KW - berninamycins
KW - TERMINAL AMIDE FORMATION
KW - GENE-CLUSTER
KW - BIOSYNTHESIS
KW - ACTIVATION
KW - METABOLITES
KW - DISCOVERY
KW - SEQUENCE
UR - http://www.scopus.com/inward/record.url?scp=85062275198&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2018.03139
DO - 10.3389/fmicb.2018.03139
M3 - Article
SN - 1664-302X
VL - 9
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 3139
ER -