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Abstract
Ammonia-oxidizing archaea (AOA) are widespread in nature and are involved in nitrification, an essential process in the global nitrogen cycle. The enzymes for ammonia oxidation and electron transport rely heavily on copper (Cu), which can be limited in nature. In this study the model soil archaeon Nitrososphaera viennensis was investigated via transcriptomic analysis to gain insight regarding possible Cu uptake mechanisms and compensation strategies when Cu becomes limiting. Upon Cu limitation, N. viennensis exhibited impaired nitrite production and thus growth, which was paralleled by downregulation of ammonia oxidation, electron transport, carbon fixation, nucleotide, and lipid biosynthesis pathway genes. Under Cu-limitation, 1547 out of 3180 detected genes were differentially expressed, with 784 genes upregulated and 763 downregulated. The most highly upregulated genes encoded proteins with a possible role in Cu binding and uptake, such as the Cu chelator and transporter CopC/D, disulfide bond oxidoreductase D (dsbD), and multicopper oxidases. While this response differs from the marine strain Nitrosopumilus maritimus, conserved sequence motifs in some of the Cu-responsive genes suggest conserved transcriptional regulation in terrestrial AOA. This study provides possible gene regulation and energy conservation mechanisms linked to Cu bioavailability and presents the first model for Cu uptake by a soil AOA.
Original language | English |
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Pages (from-to) | 2659-2674 |
Number of pages | 16 |
Journal | The ISME Journal: multidisciplinary journal of microbial ecology |
Volume | 14 |
Issue number | 11 |
Early online date | 14 Jul 2020 |
DOIs | |
Publication status | Published - Nov 2020 |
Austrian Fields of Science 2012
- 106022 Microbiology
- 105906 Environmental geosciences
Keywords
- Archaea
- AOA
- COPPER
- Transcriptome
- ammonia oxidation
- NITROSOSPHAERA-VIENNENSIS
- BACTERIA
- REDOX TRANSFORMATION
- STRESS-RESPONSE
- NITROSOPUMILUS-MARITIMUS
- CYTOCHROME-C-OXIDASE
- MULTICOPPER OXIDASES
- ARCHAEA
- COMPLETE NITRIFICATION
- SURFACE WATERS
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Dive into the research topics of 'Genome wide transcriptomic analysis of the soil ammonia oxidizing archaeon Nitrososphaera viennensis upon exposure to copper limitation'. Together they form a unique fingerprint.Projects
- 1 Finished
Activities
- 1 Talk or oral contribution
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Unraveling the role of microorganisms in biogeochemical cycles, including iron and copper cycles
Carolina Reyes (Speaker)
17 Jul 2020Activity: Talks and presentations › Talk or oral contribution › Science to Public
Research output
- 1 Article
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Genome wide transcriptomic analysis of the soil ammonia oxidizing archaeon Nitrososphaera viennensis upon exposure to copper limitation
Reyes, C., Hodgskiss, L., Kerou, M., Pribasnig, T., Abby, S., Bayer, B., Krämer, S. & Schleper, C., Nov 2020, In: The ISME Journal: multidisciplinary journal of microbial ecology. 14, 11, p. 2659-2674 16 p.Publications: Contribution to journal › Article › Peer Reviewed
Open Access