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Haplotype-resolved genome assembly of the tetraploid potato cultivar Désirée

  • Tim Godec
  • , Sebastian Beier
  • , Natalia Yaneth Rodriguez-Granados
  • , Rashmi Sasidharan
  • , Lamis Abdelhakim
  • , Markus Teige
  • , Björn Usadel
  • , Kristina Gruden
  • , Marko Petek

Publications: Contribution to journalArticlePeer Reviewed

Abstract

Cultivar Désirée is an important model for potato functional genomics studies to assist breeding strategies. Here, we present a haplotype-resolved genome assembly of Désirée, achieved by assembling PacBio HiFi reads and Hi-C scaffolding, resulting in a high-contiguity chromosome-level assembly. We implemented a comprehensive annotation pipeline incorporating gene models and functional annotations from the Solanum tuberosum Phureja DM reference genome alongside RNA-seq reads to provide high-quality gene and transcript annotations. Additionally, we provide a genome-wide DNA methylation profile using Oxford Nanopore reads, enabling insights into potato epigenetics. The assembled genome, annotations, methylation and expression data are visualised in a publicly accessible genome browser, providing a valuable resource for the potato research community.

Original languageEnglish
Article number1044
JournalScientific Data
Volume12
Issue number1
DOIs
Publication statusPublished - 20 Jun 2025

Austrian Fields of Science 2012

  • 106014 Genomics

Keywords

  • Solanum tuberosum/genetics
  • Genome, Plant
  • Haplotypes
  • Tetraploidy
  • DNA Methylation
  • Molecular Sequence Annotation

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