HPLC separation of opium alkaloids on porous and non-porous stationary phases

Liselotte Krenn, B Boros, Robert Ohmacht, Laszlo Jelinek

    Publications: Contribution to journalMeeting abstract/Conference paper

    Abstract

    A new reversed-phase (RP) HPLC method has been developed and validated for the separation of the main opium alkaloids morphine, codeine, thebaine, papaverine and noscapine on a non-porous (micropellicular) stationary phase. On this phase quantification of the compounds by internal standardization with brucine was achieved extremely rapidly, in ca 1.5 min, only. Thus, the analysis time for the opium alkaloids was approximately one tenth of that on porous stationary phases. Different opium samples were investigated using non-porous and porous packings. The correlation between the results was excellent.
    Original languageEnglish
    Pages (from-to)S175-S178
    JournalChromatographia
    Volume51
    Issue numberSUPPL. 2
    DOIs
    Publication statusPublished - 2000

    Austrian Fields of Science 2012

    • 3012 Pharmacy, Pharmacology, Toxicology

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