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Inhibiting quinolone biosynthesis of Burkholderia

  • Michaela Prothiwa
  • , Verena Filz
  • , Sebastian Oehler
  • , Thomas Boettcher (Corresponding author)

Publications: Contribution to journalArticlePeer Reviewed

Abstract

2-Alkylquinolones are important signalling molecules of Burkholderia species. We developed a substrate-based chemical probe against the central quinolone biosynthesis enzyme HmqD and applied it in competitive profiling experiments to discover the first known HmqD inhibitors. The most potent inhibitors quantitatively blocked quinolone production in Burkholderia cultures with single-digit micromolar efficacy.
Original languageEnglish
Pages (from-to)6908-6912
Number of pages5
JournalChemical Science
Volume12
Issue number20
DOIs
Publication statusPublished - 28 May 2021

Funding

We thank Prof. Dr Andreas Marx for his generous support and Malin Bein and Prof. Dr Tanja Gaich for access to mass spectrometry. We gratefully acknowledge funding by the Emmy Noether program of the Deutsche Forschungsgemeinscha. (DFG), EU FP7 Marie Curie Zukun.skolleg Incoming Fellowship Program -University of Konstanz grant no. 291784, SFB969 project C06, and Konstanz Research School Chemical Biology (KoRS-CB). MP was supported by a Zeiss PhD fellowship.

Austrian Fields of Science 2012

  • 104004 Chemical biology
  • 106023 Molecular biology

Keywords

  • HHQ
  • MOLECULES
  • N-OXIDES
  • PQS
  • PSEUDOMALLEI
  • PSEUDOMONAS-AERUGINOSA
  • SYSTEM
  • VIRULENCE

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