TY - JOUR
T1 - Lipidome Unsaturation Affects the Morphology and Proteome of the Drosophila Eye
AU - Kumar, Mukesh
AU - Has, Canan
AU - Lam-Kamath, Khanh
AU - Ayciriex, Sophie
AU - Dewett, Deepshe
AU - Bashir, Mhamed
AU - Poupault, Clara
AU - Schuhmann, Kai
AU - Thomas, Henrik
AU - Knittelfelder, Oskar
AU - Raghuraman, Bharath Kumar
AU - Ahrends, Robert
AU - Rister, Jens
AU - Shevchenko, Andrej
N1 - Publisher Copyright:
© 2024 The Authors. Published by American Chemical Society
PY - 2024/4/5
Y1 - 2024/4/5
N2 - Organisms respond to dietary and environmental challenges by altering the molecular composition of their glycerolipids and glycerophospholipids (GPLs), which may favorably adjust the physicochemical properties of lipid membranes. However, how lipidome changes affect the membrane proteome and, eventually, the physiology of specific organs is an open question. We addressed this issue in Drosophila melanogaster, which is not able to synthesize sterols and polyunsaturated fatty acids but can acquire them from food. We developed a series of semisynthetic foods to manipulate the length and unsaturation of fatty acid moieties in GPLs and singled out proteins whose abundance is specifically affected by membrane lipid unsaturation in the Drosophila eye. Unexpectedly, we identified a group of proteins that have muscle-related functions and increased their abundances under unsaturated eye lipidome conditions. In contrast, the abundance of two stress response proteins, Turandot A and Smg5, is decreased by lipid unsaturation. Our findings could guide the genetic dissection of homeostatic mechanisms that maintain visual function when the eye is exposed to environmental and dietary challenges.
AB - Organisms respond to dietary and environmental challenges by altering the molecular composition of their glycerolipids and glycerophospholipids (GPLs), which may favorably adjust the physicochemical properties of lipid membranes. However, how lipidome changes affect the membrane proteome and, eventually, the physiology of specific organs is an open question. We addressed this issue in Drosophila melanogaster, which is not able to synthesize sterols and polyunsaturated fatty acids but can acquire them from food. We developed a series of semisynthetic foods to manipulate the length and unsaturation of fatty acid moieties in GPLs and singled out proteins whose abundance is specifically affected by membrane lipid unsaturation in the Drosophila eye. Unexpectedly, we identified a group of proteins that have muscle-related functions and increased their abundances under unsaturated eye lipidome conditions. In contrast, the abundance of two stress response proteins, Turandot A and Smg5, is decreased by lipid unsaturation. Our findings could guide the genetic dissection of homeostatic mechanisms that maintain visual function when the eye is exposed to environmental and dietary challenges.
KW - diet
KW - Drosophila
KW - membrane lipid unsaturation
KW - phototransduction
KW - proteome
KW - retina
UR - http://www.scopus.com/inward/record.url?scp=85188008571&partnerID=8YFLogxK
U2 - 10.1021/acs.jproteome.3c00570
DO - 10.1021/acs.jproteome.3c00570
M3 - Article
C2 - 38484338
AN - SCOPUS:85188008571
SN - 1535-3893
VL - 23
SP - 1188
EP - 1199
JO - Journal of Proteome Research
JF - Journal of Proteome Research
IS - 4
ER -