Mass Western for absolute quantification of target proteins and considerations about the instrument of choice.

    Publications: Contribution to bookChapter

    Abstract

    The Mass Western describes the absolute quantification of proteins based on stable isotope labeled integral standard peptides and liquid chromatography coupled selective reaction monitoring triple quadrupole mass spectrometry (LC-SRM/MS). Here, we present a detailed workflow including tips and we discuss advantages and disadvantages of using different types of MS for absolute quantification.
    Original languageEnglish
    Title of host publicationPlant Proteomics
    Subtitle of host publicationMethods in Molecular Biology
    PublisherHumana Press, Inc.
    Pages199-208
    Number of pages10
    Volume1072
    ISBN (Electronic)978-1-62703-631-3
    ISBN (Print)978-1-62703-630-6
    DOIs
    Publication statusPublished - 2014

    Publication series

    SeriesMethods in Molecular Biology
    Volume1072
    ISSN1064-3745

    Austrian Fields of Science 2012

    • 106037 Proteomics
    • 106023 Molecular biology
    • 106005 Bioinformatics
    • 106014 Genomics

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