Microscopic and Proteomic Analysis of Dissected Developing Barley Endosperm Layers Reveals the Starchy Endosperm as Prominent Storage Tissue for ER-Derived Hordeins Alongside the Accumulation of Barley Protein Disulfide Isomerase (HvPDIL1-1)

  • Valentin Roustan
  • , Pierre-Jean Roustan
  • , Marieluise Weidinger
  • , Siegfried Reipert
  • , Eszter Kapusi
  • , Azita Shabrangy
  • , Eva Stoger
  • , Wolfram Weckwerth
  • , Verena Ibl

    Publications: Contribution to journal › Article › Peer Reviewed

    Original languageEnglish
    Article number1248
    Number of pages23
    JournalFrontiers in Plant Science
    Volume9
    DOIs
    Publication statusPublished - 10 Sept 2018

    Funding

    We thank Dr. Karl Schedle from the Institute of Animal Nutrition, Livestock Products, and Nutrition Physiology (TTE), University of Natural Resources and Life Sciences, Vienna, for sharing the RNA lab and Christiane Schwartz for RTqPCR support. We thank the BOKU-VIBT Imaging-Center and Dr. Monika Debreczeny for help with LMD. The authors acknowledge Dr. Richard Strasser, Department of Applied Genetics and Cell Biology (DAGZ) of the University of Natural Resources and Life Sciences, Vienna, for kindly providing antiPDI antisera. We thank IPK Gatersleben for kindly providing us the GP barley seeds. We are grateful to Mag. Helga Kudler for carefully reading the manuscript. This work was financially supported by the Austrian Science Fund FWF (P29454-B22 and P 29303-B22).

    Austrian Fields of Science 2012

    • 106008 Botany

    Keywords

    • shotgun proteomics
    • confocal microscopy
    • seed storage proteins
    • barley
    • PDIL1-1
    • grain development
    • tissue-specific
    • protein bodies
    • DEVELOPING WHEAT-GRAIN
    • ENDOPLASMIC-RETICULUM
    • ARABIDOPSIS-THALIANA
    • GLUTEN PROTEINS
    • GENE-EXPRESSION
    • FAMILY PROTEINS
    • RICE ENDOSPERM
    • BOND FORMATION
    • BODY FORMATION
    • CEREAL SEEDS
    • Confocal microscopy
    • Barley
    • Shotgun proteomics
    • Grain development
    • Protein bodies
    • Seed storage proteins
    • Tissue-specific

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