Molecular dimensions and structural features of neutral polysaccharides from the seed mucilage of Hyptis suaveolens L.

Werner Praznik; Andrea Cavarkapa; Frank M. Unger; Renate Loeppert; Wolfgang Holzer; Helmut Viernstein; Monika Mueller

doi:10.1016/j.foodchem.2016.11.087

Molecular dimensions and structural features of neutral polysaccharides from the seed mucilage of Hyptis suaveolens L.

Werner Praznik (Corresponding author), Andrea Cavarkapa, Frank M. Unger, Renate Loeppert, Wolfgang Holzer, Helmut Viernstein, Monika Mueller

Publications: Contribution to journal › Article › Peer Reviewed

Abstract

The seed mucilage of Hyptis suaveolens L. includes acid – and neutral heteropolysaccharides in a ratio of
about 1:1. The anionic charged fraction responsible for swelling and viscous behaviour possesses an average
molar mass of Mw = 350 kg/mol, Mn = 255 kg/mol. The neutral polysaccharide fraction shows an
average molar mass of Mw = 47 kg/mol and Mn = 28 kg/mol and is composed of D-Galp-, D-Glcp- and
D-Manp residues in a molar ratio of about 3:2:1. The structural features present galactoglucan (30%) and
galactoglucomannan (70%) with a high level of terminal b-linked D-Galp residues (18%). Structural details
of galactoglucomannan are derived by combined enzymatic and chemical methods as well as NMR
spectroscopy. Sequences of octa/nonasaccharide b-D-Glcp-(1 ?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-
b-D-Manp-(1?4)-b-D-Glcp-(1?4)-b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-D-Manp and
lower mass tetrasaccharide repeating units b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-DManp
were found. The level of the prebiotic activity is related to the availability of b-linked D-Galp residues
in the side chains of the molecules.

Original language	English
Pages (from-to)	1997-2004
Number of pages	8
Journal	Food Chemistry
Volume	221
DOIs	https://doi.org/10.1016/j.foodchem.2016.11.087
Publication status	Published - 15 Apr 2017

Austrian Fields of Science 2012

301208 Pharmaceutical technology
104013 Natural product chemistry

Keywords

Galactoglucan
Galactoglucomannan
Hyptis suaveolens L.
Methylation analysis
Mucilage
NMR spectroscopy
Polysaccharide
GALACTOGLUCOMANNAN

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10.1016/j.foodchem.2016.11.087

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@article{551f1464754a42e385b29e945505b8f3,

title = "Molecular dimensions and structural features of neutral polysaccharides from the seed mucilage of Hyptis suaveolens L.",

abstract = "The seed mucilage of Hyptis suaveolens L. includes acid – and neutral heteropolysaccharides in a ratio ofabout 1:1. The anionic charged fraction responsible for swelling and viscous behaviour possesses an averagemolar mass of Mw = 350 kg/mol, Mn = 255 kg/mol. The neutral polysaccharide fraction shows anaverage molar mass of Mw = 47 kg/mol and Mn = 28 kg/mol and is composed of D-Galp-, D-Glcp- andD-Manp residues in a molar ratio of about 3:2:1. The structural features present galactoglucan (30%) andgalactoglucomannan (70%) with a high level of terminal b-linked D-Galp residues (18%). Structural detailsof galactoglucomannan are derived by combined enzymatic and chemical methods as well as NMRspectroscopy. Sequences of octa/nonasaccharide b-D-Glcp-(1 ?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-D-Manp-(1?4)-b-D-Glcp-(1?4)-b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-D-Manp andlower mass tetrasaccharide repeating units b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-DManpwere found. The level of the prebiotic activity is related to the availability of b-linked D-Galp residuesin the side chains of the molecules.",

keywords = "Galactoglucan, Galactoglucomannan, Hyptis suaveolens L., Methylation analysis, Mucilage, NMR spectroscopy, Polysaccharide, GALACTOGLUCOMANNAN",

author = "Werner Praznik and Andrea Cavarkapa and Unger, {Frank M.} and Renate Loeppert and Wolfgang Holzer and Helmut Viernstein and Monika Mueller",

year = "2017",

month = apr,

day = "15",

doi = "10.1016/j.foodchem.2016.11.087",

language = "English",

volume = "221",

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journal = "Food Chemistry",

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TY - JOUR

T1 - Molecular dimensions and structural features of neutral polysaccharides from the seed mucilage of Hyptis suaveolens L.

AU - Praznik, Werner

AU - Cavarkapa, Andrea

AU - Unger, Frank M.

AU - Loeppert, Renate

AU - Holzer, Wolfgang

AU - Viernstein, Helmut

AU - Mueller, Monika

PY - 2017/4/15

Y1 - 2017/4/15

N2 - The seed mucilage of Hyptis suaveolens L. includes acid – and neutral heteropolysaccharides in a ratio ofabout 1:1. The anionic charged fraction responsible for swelling and viscous behaviour possesses an averagemolar mass of Mw = 350 kg/mol, Mn = 255 kg/mol. The neutral polysaccharide fraction shows anaverage molar mass of Mw = 47 kg/mol and Mn = 28 kg/mol and is composed of D-Galp-, D-Glcp- andD-Manp residues in a molar ratio of about 3:2:1. The structural features present galactoglucan (30%) andgalactoglucomannan (70%) with a high level of terminal b-linked D-Galp residues (18%). Structural detailsof galactoglucomannan are derived by combined enzymatic and chemical methods as well as NMRspectroscopy. Sequences of octa/nonasaccharide b-D-Glcp-(1 ?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-D-Manp-(1?4)-b-D-Glcp-(1?4)-b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-D-Manp andlower mass tetrasaccharide repeating units b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-DManpwere found. The level of the prebiotic activity is related to the availability of b-linked D-Galp residuesin the side chains of the molecules.

AB - The seed mucilage of Hyptis suaveolens L. includes acid – and neutral heteropolysaccharides in a ratio ofabout 1:1. The anionic charged fraction responsible for swelling and viscous behaviour possesses an averagemolar mass of Mw = 350 kg/mol, Mn = 255 kg/mol. The neutral polysaccharide fraction shows anaverage molar mass of Mw = 47 kg/mol and Mn = 28 kg/mol and is composed of D-Galp-, D-Glcp- andD-Manp residues in a molar ratio of about 3:2:1. The structural features present galactoglucan (30%) andgalactoglucomannan (70%) with a high level of terminal b-linked D-Galp residues (18%). Structural detailsof galactoglucomannan are derived by combined enzymatic and chemical methods as well as NMRspectroscopy. Sequences of octa/nonasaccharide b-D-Glcp-(1 ?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-D-Manp-(1?4)-b-D-Glcp-(1?4)-b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-D-Manp andlower mass tetrasaccharide repeating units b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-DManpwere found. The level of the prebiotic activity is related to the availability of b-linked D-Galp residuesin the side chains of the molecules.

KW - Galactoglucan

KW - Galactoglucomannan

KW - Hyptis suaveolens L.

KW - Methylation analysis

KW - Mucilage

KW - NMR spectroscopy

KW - Polysaccharide

KW - GALACTOGLUCOMANNAN

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U2 - 10.1016/j.foodchem.2016.11.087

DO - 10.1016/j.foodchem.2016.11.087

M3 - Article

AN - SCOPUS:85006778773

SN - 0308-8146

VL - 221

SP - 1997

EP - 2004

JO - Food Chemistry

JF - Food Chemistry

ER -

Molecular dimensions and structural features of neutral polysaccharides from the seed mucilage of Hyptis suaveolens L.

Abstract

Austrian Fields of Science 2012

Keywords

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