Neuroactive metabolites and bile acids are altered in extremely premature infants with brain injury

Manuel Pristner; Daniel Wasinger; David Seki; Katrin Klebermaß-Schrehof; Angelika Berger; David Berry; Lukas Wisgrill; Benedikt Warth

doi:10.1016/j.xcrm.2024.101480

Neuroactive metabolites and bile acids are altered in extremely premature infants with brain injury

Manuel Pristner, Daniel Wasinger, David Seki, Katrin Klebermaß-Schrehof, Angelika Berger, David Berry, Lukas Wisgrill, Benedikt Warth

Publications: Contribution to journal › Article › Peer Reviewed

Abstract

The gut microbiome is associated with pathological neurophysiological evolvement in extremely premature infants suffering from brain injury. The exact underlying mechanism and its associated metabolic signatures in infants are not fully understood. To decipher metabolite profiles linked to neonatal brain injury, we investigate the fecal and plasma metabolome of samples obtained from a cohort of 51 extremely premature infants at several time points, using liquid chromatography (LC)-high-resolution mass spectrometry (MS)-based untargeted metabolomics and LC-MS/MS-based targeted analysis for investigating bile acids and amidated bile acid conjugates. The data are integrated with 16S rRNA gene amplicon gut microbiome profiles as well as patient cytokine, growth factor, and T cell profiles. We find an early onset of differentiation in neuroactive metabolites between infants with and without brain injury. We detect several bacterially derived bile acid amino acid conjugates in plasma and feces. These results provide insights into the early-life metabolome of extremely premature infants.

Original language	English
Article number	101480
Number of pages	24
Journal	Cell Reports Medicine
Volume	5
Issue number	4
DOIs	https://doi.org/10.1016/j.xcrm.2024.101480
Publication status	Published - 16 Apr 2024

Austrian Fields of Science 2012

106026 Ecosystem research
106022 Microbiology
106059 Microbiome research

Keywords

bile acid amino acid conjugates
extremely premature infant
gut-immune-brain axis
neonatal brain injury
untargeted metabolomics

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https://phaidra.univie.ac.at/o:2085411Licence: CC BY 4.0

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title = "Neuroactive metabolites and bile acids are altered in extremely premature infants with brain injury",

abstract = "The gut microbiome is associated with pathological neurophysiological evolvement in extremely premature infants suffering from brain injury. The exact underlying mechanism and its associated metabolic signatures in infants are not fully understood. To decipher metabolite profiles linked to neonatal brain injury, we investigate the fecal and plasma metabolome of samples obtained from a cohort of 51 extremely premature infants at several time points, using liquid chromatography (LC)-high-resolution mass spectrometry (MS)-based untargeted metabolomics and LC-MS/MS-based targeted analysis for investigating bile acids and amidated bile acid conjugates. The data are integrated with 16S rRNA gene amplicon gut microbiome profiles as well as patient cytokine, growth factor, and T cell profiles. We find an early onset of differentiation in neuroactive metabolites between infants with and without brain injury. We detect several bacterially derived bile acid amino acid conjugates in plasma and feces. These results provide insights into the early-life metabolome of extremely premature infants.",

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AU - Pristner, Manuel

AU - Wasinger, Daniel

AU - Seki, David

AU - Klebermaß-Schrehof, Katrin

AU - Berger, Angelika

AU - Berry, David

AU - Wisgrill, Lukas

AU - Warth, Benedikt

PY - 2024/4/16

Y1 - 2024/4/16

N2 - The gut microbiome is associated with pathological neurophysiological evolvement in extremely premature infants suffering from brain injury. The exact underlying mechanism and its associated metabolic signatures in infants are not fully understood. To decipher metabolite profiles linked to neonatal brain injury, we investigate the fecal and plasma metabolome of samples obtained from a cohort of 51 extremely premature infants at several time points, using liquid chromatography (LC)-high-resolution mass spectrometry (MS)-based untargeted metabolomics and LC-MS/MS-based targeted analysis for investigating bile acids and amidated bile acid conjugates. The data are integrated with 16S rRNA gene amplicon gut microbiome profiles as well as patient cytokine, growth factor, and T cell profiles. We find an early onset of differentiation in neuroactive metabolites between infants with and without brain injury. We detect several bacterially derived bile acid amino acid conjugates in plasma and feces. These results provide insights into the early-life metabolome of extremely premature infants.

AB - The gut microbiome is associated with pathological neurophysiological evolvement in extremely premature infants suffering from brain injury. The exact underlying mechanism and its associated metabolic signatures in infants are not fully understood. To decipher metabolite profiles linked to neonatal brain injury, we investigate the fecal and plasma metabolome of samples obtained from a cohort of 51 extremely premature infants at several time points, using liquid chromatography (LC)-high-resolution mass spectrometry (MS)-based untargeted metabolomics and LC-MS/MS-based targeted analysis for investigating bile acids and amidated bile acid conjugates. The data are integrated with 16S rRNA gene amplicon gut microbiome profiles as well as patient cytokine, growth factor, and T cell profiles. We find an early onset of differentiation in neuroactive metabolites between infants with and without brain injury. We detect several bacterially derived bile acid amino acid conjugates in plasma and feces. These results provide insights into the early-life metabolome of extremely premature infants.

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KW - gut-immune-brain axis

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Neuroactive metabolites and bile acids are altered in extremely premature infants with brain injury

Abstract

Austrian Fields of Science 2012

Keywords

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