TY - JOUR
T1 - Oligonucleotide microarray for 16S rRNA gene-based detection of all recognized lineages of sulfate-reducing prokaryotes in the environment
AU - Loy, Alexander
AU - Lehner, Angelika
AU - Adamczyk, Justyna
AU - Meier, Harald
AU - Ernst, Jens
AU - Schleifer, Karl-Heinz
AU - Wagner, Michael
N1 - Zeitschrift: Applied and Environmental Microbiology
DOI: 10.1128/AEM.68.10.5064-5081.2002
Coden: AEMID
Affiliations: Lehrstuhl fur Mikrobiologie, Technische Universitat Munchen, Am Hochanger 4, D-85350 Freising, Germany
Adressen: Wagner, M.; Lehrstuhl fur Mikrobiologie; Technische Universitat Munchen; Am Hochanger 4 D-85350 Freising, Germany; email: [email protected]
Import aus Scopus: 2-s2.0-0036795787
24.08.2007: Datenanforderung 1832 (Import Sachbearbeiter)
PY - 2002
Y1 - 2002
N2 - For cultivation-independent detection of sulfate-reducing prokaryotes (SRPs) an oligonucleotide microarray consisting of 132 16S rRNA gene-targeted oligonucleotide probes (18-mers) having hierarchical and parallel (identical) specificity for the detection of all known lineages of sulfate-reducing prokaryotes (SRP-PhyloChip) was designed and subsequently evaluated with 41 suitable pure cultures of SRPs. The applicability of SRP-PhyloChip for diversity screening of SRPs in environmental and clinical samples was tested by using samples from periodontal tooth pockets and from the chemocline of a hypersaline cyanobacterial mat from Solar Lake (Sinai, Egypt). Consistent with previous studies, SRP-PhyloChip indicated the occurrence of Desulfomicrobium spp. in the tooth pockets and the presence of Desulfonema- and Desulfomonile-like SRPs (together with other SRPs) in the chemocline of the mat. The SRP-PhyloChip results were confirmed by several DNA microarray-independent techniques, including specific PCR amplification, cloning, and sequencing of SRP 16S rRNA genes and the genes encoding the dissimilatory (bi)sulfite reductase (dsrAB).
AB - For cultivation-independent detection of sulfate-reducing prokaryotes (SRPs) an oligonucleotide microarray consisting of 132 16S rRNA gene-targeted oligonucleotide probes (18-mers) having hierarchical and parallel (identical) specificity for the detection of all known lineages of sulfate-reducing prokaryotes (SRP-PhyloChip) was designed and subsequently evaluated with 41 suitable pure cultures of SRPs. The applicability of SRP-PhyloChip for diversity screening of SRPs in environmental and clinical samples was tested by using samples from periodontal tooth pockets and from the chemocline of a hypersaline cyanobacterial mat from Solar Lake (Sinai, Egypt). Consistent with previous studies, SRP-PhyloChip indicated the occurrence of Desulfomicrobium spp. in the tooth pockets and the presence of Desulfonema- and Desulfomonile-like SRPs (together with other SRPs) in the chemocline of the mat. The SRP-PhyloChip results were confirmed by several DNA microarray-independent techniques, including specific PCR amplification, cloning, and sequencing of SRP 16S rRNA genes and the genes encoding the dissimilatory (bi)sulfite reductase (dsrAB).
M3 - Article
SN - 0099-2240
VL - 68
SP - 5064
EP - 5081
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 10
ER -