pEPito: a significantly improved non-viral episomal expression vector for mammalian cells

Rudolf Haase; Orestis Argyros; Suet-Ping Wong; Richard P. Harbottle; Hans J. Lipps; Manfred Ogris; Terese Magnusson; Maria G. Vizoso Pinto; Juergen Haas; Armin Baiker

doi:10.1186/1472-6750-10-20

pEPito: a significantly improved non-viral episomal expression vector for mammalian cells

Rudolf Haase
, Orestis Argyros
, Suet-Ping Wong
, Richard P. Harbottle
, Hans J. Lipps
, Manfred Ogris
, Terese Magnusson
, Maria G. Vizoso Pinto
, Juergen Haas
, Armin Baiker

Publications: Contribution to journal › Article › Peer Reviewed

Original language	English
Article number	20
Number of pages	14
Journal	BMC Biotechnology
Volume	10
DOIs	https://doi.org/10.1186/1472-6750-10-20
Publication status	Published - 15 Mar 2010
Externally published	Yes

Funding

Financial support by the Deutsche Forschungsgemeinschaft (BA 2035/3-1 and SPP1230 priority program "Mechanisms of gene vector entry and persistence") and the Bundesministerium fuer Bildung und Forschung (BMBF BioChancePLUS/FKZ: 0315182) to AB is gratefully acknowledged. We kindly acknowledge Michael Kahn for providing E. coli DB3.1 lambda pir. Furthermore we like to thank Helga Mairhofer and Georg Malterer for their help with real time PCR and Anja Ehrhardt for her help with Southern blotting.

Austrian Fields of Science 2012

301207 Pharmaceutical chemistry

Keywords

SCAFFOLD/MATRIX-ATTACHED REGION
GREEN-FLUORESCENT PROTEIN
MOUSE OOCYTE MEIOSIS
PLASMID DNA VECTORS
TRANSGENE EXPRESSION
GENE-EXPRESSION
IN-VIVO
LONG-TERM
HISTONE ACETYLATION
NUCLEAR-MATRIX

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10.1186/1472-6750-10-20Licence: Other

Cite this

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title = "pEPito: a significantly improved non-viral episomal expression vector for mammalian cells",

keywords = "SCAFFOLD/MATRIX-ATTACHED REGION, GREEN-FLUORESCENT PROTEIN, MOUSE OOCYTE MEIOSIS, PLASMID DNA VECTORS, TRANSGENE EXPRESSION, GENE-EXPRESSION, IN-VIVO, LONG-TERM, HISTONE ACETYLATION, NUCLEAR-MATRIX",

author = "Rudolf Haase and Orestis Argyros and Suet-Ping Wong and Harbottle, \{Richard P.\} and Lipps, \{Hans J.\} and Manfred Ogris and Terese Magnusson and Pinto, \{Maria G. Vizoso\} and Juergen Haas and Armin Baiker",

year = "2010",

month = mar,

day = "15",

doi = "10.1186/1472-6750-10-20",

language = "English",

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TY - JOUR

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T2 - a significantly improved non-viral episomal expression vector for mammalian cells

AU - Haase, Rudolf

AU - Argyros, Orestis

AU - Wong, Suet-Ping

AU - Harbottle, Richard P.

AU - Lipps, Hans J.

AU - Ogris, Manfred

AU - Magnusson, Terese

AU - Pinto, Maria G. Vizoso

AU - Haas, Juergen

AU - Baiker, Armin

PY - 2010/3/15

Y1 - 2010/3/15

KW - SCAFFOLD/MATRIX-ATTACHED REGION

KW - GREEN-FLUORESCENT PROTEIN

KW - MOUSE OOCYTE MEIOSIS

KW - PLASMID DNA VECTORS

KW - TRANSGENE EXPRESSION

KW - GENE-EXPRESSION

KW - IN-VIVO

KW - LONG-TERM

KW - HISTONE ACETYLATION

KW - NUCLEAR-MATRIX

U2 - 10.1186/1472-6750-10-20

DO - 10.1186/1472-6750-10-20

M3 - Article

SN - 1472-6750

SN - 1876-4347

VL - 10

JO - BMC Biotechnology

JF - BMC Biotechnology

M1 - 20

ER -

pEPito: a significantly improved non-viral episomal expression vector for mammalian cells

Funding

Austrian Fields of Science 2012

Keywords

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