TY - JOUR
T1 - Phylogenetic analysis of and oligonucleotide probe development for Eikelboom type 021N filamentous bacteria isolated from bulking activated sludge
AU - Kanagawa, Takahiro
AU - Kamagata, Y
AU - Aruga, Shinobu
AU - Kohno, Tetsuro
AU - Horn, Matthias
AU - Wagner, Michael
N1 - Zeitschrift: Applied and Environmental Microbiology
DOI: 10.1128/AEM.66.11.5043-5052.2000
Coden: AEMID
Affiliations: Natl. Inst. of Biosci./Hum.-Technol., Agency of Industrial Sci./Technology, 1-1 Higashi, Tsukuba 305-8566, Japan
Adressen: Kanagawa, T.; Natl. Inst. of Biosci./Hum.-Technol.; Agency of Industrial Sci./Technology; 1-1 Higashi Tsukuba 305-8566, Japan; email: [email protected]
Import aus Scopus: 2-s2.0-0033754288
24.08.2007: Datenanforderung 1832 (Import Sachbearbeiter)
PY - 2000
Y1 - 2000
N2 - Fifteen filamentous strains, morphologically classified as Eikelboom type 021N bacteria, were isolated from bulking activated sludges. Based on comparative 16S ribosomal DNA (rDNA) sequence analysis, all strains form a monophyletic cluster together with all recognized Thiothrix species (88.3 to 98.7% 16S rDNA sequence similarity) within the gamma-subclass of Proteobacteria. The investigated Eikelboom type 021N isolates were subdivided into three distinct groups (I to III) demonstrating a previously unrecognized genetic diversity hidden behind the uniform morphology of the filaments. For in situ detection of these bacteria, 16S rRNA-targeted oligonucleotide probes specific for the entire Eikelboom type 021N-Thiothrix cluster and the Eikelboom type 021N groups I, II, and III, respectively, were designed, evaluated, and successfully applied in activated sludge.
AB - Fifteen filamentous strains, morphologically classified as Eikelboom type 021N bacteria, were isolated from bulking activated sludges. Based on comparative 16S ribosomal DNA (rDNA) sequence analysis, all strains form a monophyletic cluster together with all recognized Thiothrix species (88.3 to 98.7% 16S rDNA sequence similarity) within the gamma-subclass of Proteobacteria. The investigated Eikelboom type 021N isolates were subdivided into three distinct groups (I to III) demonstrating a previously unrecognized genetic diversity hidden behind the uniform morphology of the filaments. For in situ detection of these bacteria, 16S rRNA-targeted oligonucleotide probes specific for the entire Eikelboom type 021N-Thiothrix cluster and the Eikelboom type 021N groups I, II, and III, respectively, were designed, evaluated, and successfully applied in activated sludge.
M3 - Article
SN - 0099-2240
VL - 66
SP - 5043
EP - 5052
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 11
ER -