Phytochemical composition of Potentilla anserina L. analyzed by an integrative GC-MS and LC-MS metabolomics platform

Angela Mari, David Lyon, Lena Fragner, Paola Montoro, Sonia Piacente (Corresponding author), Stefanie Wienkoop, Volker Egelhofer, Wolfram Weckwerth (Corresponding author)

    Publications: Contribution to journalArticlePeer Reviewed

    Abstract

    Potentilla anserina L. (Rosaceae) is known for its beneficial effects of prevention of pre-menstrual syndrome (PMS). For this reason P. anserina is processed into many food supplements and pharmaceutical preparations. Here we analyzed hydroalcoholic reference extracts and compared them with various extracts of different pharmacies using an integrative metabolomics platform comprising GC-MS and LC-MS analysis and software toolboxes for data alignment (MetMAX Beta 1.0) and multivariate statistical analysis (COVAIN 1.0). Multivariate statistics of the integrated GC-MS and LC-MS data showed strong differences between the different plant extract formulations. Different groups of compounds such as chlorogenic acid, kaempferol 3-O-rutinoside, acacetin 7-O-rutinoside, and genistein were reported for the first time in this species. The typical fragmentation pathway of the isoflavone genistein confirmed the identification of this active compound that was present with different abundances in all the extracts analyzed. As a result we have revealed that different extraction procedures from different vendors produce different chemical compositions, e.g. different genistein concentrations. Consequently, the treatment may have different effects. The integrative metabolomics platform provides the highest resolution of the phytochemical composition and a mean to define subtle differences in plant extract formulations.
    Original languageEnglish
    Pages (from-to)599-607
    Number of pages9
    JournalMetabolomics
    Volume9
    Issue number3
    DOIs
    Publication statusPublished - Jun 2013

    Austrian Fields of Science 2012

    • 1030 Physics, Astronomy
    • 106034 Phytochemistry
    • 1040 Chemistry

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