Proposing a validation scheme for C-13 metabolite tracer studies in high-resolution mass spectrometry

Michaela Schwaiger-Haber; Gerrit Hermann; Yasin El Abiead; Evelyn Rampler; Stefanie Wernisch; Kelli Sas; Subramaniam Pennathur; Gunda Koellensperger

doi:10.1007/s00216-019-01773-7

Proposing a validation scheme for C-13 metabolite tracer studies in high-resolution mass spectrometry

Michaela Schwaiger-Haber
, Gerrit Hermann
, Yasin El Abiead
, Evelyn Rampler
, Stefanie Wernisch
, Kelli Sas
, Subramaniam Pennathur
, Gunda Koellensperger

Publications: Contribution to journal › Article › Peer Reviewed

Original language	English
Pages (from-to)	3103-3113
Number of pages	11
Journal	Analytical and Bioanalytical Chemistry
Volume	411
Issue number	14
DOIs	https://doi.org/10.1007/s00216-019-01773-7
Publication status	Published - May 2019

Funding

Open access funding provided by University of Vienna. The Mass Spectrometry Center (MSC), Faculty of Chemistry, University of Vienna is acknowledged for providing mass spectrometric instrumentation. Mate Rusz is acknowledged for providing the HCT 116 cells. We thank Alexander Makarov for useful discussions regarding orbitrap data generation. This research was supported in part by a Postdoctoral Translational Science Program from the Michigan Institute for Clinical and Health Research UL1TR000433 (to S.W.) and National Institutes of Health grants P30DK089503, DK082841, P30DK081943, U2C ES026553 and DK097153 (to S.P.).

Austrian Fields of Science 2012

104026 Spectroscopy
104002 Analytical chemistry

Keywords

Metabolomics
Stable isotope tracer experiments
Stable isotope labeling experiments
Mass spectrometry
Carbon isotopologue distribution
C-13 labeling
POWERFUL TOOL
CHROMATOGRAPHY
SELECTIVITY
FLUX
QUANTIFICATION
METABOLOMICS
ISOTOPOLOGUE
PATTERNS
COVERAGE
TRACKING
C labeling

Access to Document

10.1007/s00216-019-01773-7Licence: CC BY 4.0

http://phaidra.univie.ac.at/o:1070223Licence: CC BY 4.0

Cite this

@article{593836ab80b0419f91a15db5effa06c2,

title = "Proposing a validation scheme for C-13 metabolite tracer studies in high-resolution mass spectrometry",

keywords = "Metabolomics, Stable isotope tracer experiments, Stable isotope labeling experiments, Mass spectrometry, Carbon isotopologue distribution, C-13 labeling, POWERFUL TOOL, CHROMATOGRAPHY, SELECTIVITY, FLUX, QUANTIFICATION, METABOLOMICS, ISOTOPOLOGUE, PATTERNS, COVERAGE, TRACKING, C labeling",

author = "Michaela Schwaiger-Haber and Gerrit Hermann and \{El Abiead\}, Yasin and Evelyn Rampler and Stefanie Wernisch and Kelli Sas and Subramaniam Pennathur and Gunda Koellensperger",

note = "Publisher Copyright: {\textcopyright} 2019, The Author(s).",

year = "2019",

month = may,

doi = "10.1007/s00216-019-01773-7",

language = "English",

volume = "411",

pages = "3103--3113",

journal = "Analytical and Bioanalytical Chemistry",

issn = "1618-2642",

publisher = "Springer-Verlag Berlin-Heidelberg",

number = "14",

}

TY - JOUR

T1 - Proposing a validation scheme for C-13 metabolite tracer studies in high-resolution mass spectrometry

AU - Schwaiger-Haber, Michaela

AU - Hermann, Gerrit

AU - El Abiead, Yasin

AU - Rampler, Evelyn

AU - Wernisch, Stefanie

AU - Sas, Kelli

AU - Pennathur, Subramaniam

AU - Koellensperger, Gunda

PY - 2019/5

Y1 - 2019/5

KW - Metabolomics

KW - Stable isotope tracer experiments

KW - Stable isotope labeling experiments

KW - Mass spectrometry

KW - Carbon isotopologue distribution

KW - C-13 labeling

KW - POWERFUL TOOL

KW - CHROMATOGRAPHY

KW - SELECTIVITY

KW - FLUX

KW - QUANTIFICATION

KW - METABOLOMICS

KW - ISOTOPOLOGUE

KW - PATTERNS

KW - COVERAGE

KW - TRACKING

KW - C labeling

UR - https://www.scopus.com/pages/publications/85065976092

U2 - 10.1007/s00216-019-01773-7

DO - 10.1007/s00216-019-01773-7

M3 - Article

SN - 1618-2642

VL - 411

SP - 3103

EP - 3113

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

IS - 14

ER -

Proposing a validation scheme for C-13 metabolite tracer studies in high-resolution mass spectrometry

Funding

Austrian Fields of Science 2012

Keywords

Access to Document

Other files and links

Fingerprint

Cite this