Protocol for Enrichment of the Membrane Proteome of Mature Tomato Pollen.

Puneet Paul; Palak Chaturvedi; Anida Mesihovic; Arindam Ghatak; Wolfram Weckwerth; Enrico Schleiff

doi:10.21769/BioProtoc.2315

Protocol for Enrichment of the Membrane Proteome of Mature Tomato Pollen.

Puneet Paul, Palak Chaturvedi, Anida Mesihovic, Arindam Ghatak, Wolfram Weckwerth, Enrico Schleiff

Publications: Contribution to journal › Article › Peer Reviewed

Abstract

We established and elaborated on a method to enrich the membrane proteome of mature pollen from economically relevant crop using the example of Solanum lycopersicum (tomato). To isolate the pollen protein fraction enriched in membrane proteins, a high salt concentration (750 mM of sodium chloride) was used. The membrane protein-enriched fraction was then subjected to shotgun proteomics for identification of proteins, followed by in silico analysis to annotate and classify the detected proteins.

Original language	English
Article number	e2315
Number of pages	9
Journal	Bio-Protocol
Volume	7
Issue number	11
DOIs	https://doi.org/10.21769/BioProtoc.2315
Publication status	Published - 5 Jun 2017

Austrian Fields of Science 2012

106002 Biochemistry
106037 Proteomics

Keywords

Membrane proteome
Pollen
Tomato
Proteomics

Access to Document

10.21769/BioProtoc.2315

Cite this

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abstract = "We established and elaborated on a method to enrich the membrane proteome of mature pollen from economically relevant crop using the example of Solanum lycopersicum (tomato). To isolate the pollen protein fraction enriched in membrane proteins, a high salt concentration (750 mM of sodium chloride) was used. The membrane protein-enriched fraction was then subjected to shotgun proteomics for identification of proteins, followed by in silico analysis to annotate and classify the detected proteins. ",

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AU - Mesihovic, Anida

AU - Ghatak, Arindam

AU - Weckwerth, Wolfram

AU - Schleiff, Enrico

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KW - Membrane proteome

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KW - Tomato

KW - Proteomics

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DO - 10.21769/BioProtoc.2315

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