Quantification of aquatic viruses by flow cytometry

Corina P. D. Brussaard; Jerome P. Payet; Christian Winter; Markus Weinbauer

Quantification of aquatic viruses by flow cytometry

Corina P. D. Brussaard (Corresponding author), Jerome P. Payet, Christian Winter, Markus Weinbauer

Functional and Evolutionary Ecology

Publications: Contribution to book › Chapter

Abstract

For many laboratories, flow cytometry is becoming the routine method for quantifying viruses in aquatic systems because of its high reproducibility, high sample throughput, and ability to distinguish several subpopulations of viruses. Comparison of viral counts between flow cytometry and epifluorescence microscopy typically shows slopes that are statistically not distinguishable from 1, thus confirming the usefulness of flow cytometry. Here we describe in detail all steps in the procedure, discuss potential problems, and offer solutions.

Original language	English
Title of host publication	Manual of Aquatic Viral Ecology
Editors	S. W. Wilhelm, M. G. Weinbauer, C. A. Suttle
Publisher	Unknown publisher
Pages	102-109
Number of pages	8
Publication status	Published - 2010

Austrian Fields of Science 2012

106022 Microbiology

Cite this

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title = "Quantification of aquatic viruses by flow cytometry",

abstract = "For many laboratories, flow cytometry is becoming the routine method for quantifying viruses in aquatic systems because of its high reproducibility, high sample throughput, and ability to distinguish several subpopulations of viruses. Comparison of viral counts between flow cytometry and epifluorescence microscopy typically shows slopes that are statistically not distinguishable from 1, thus confirming the usefulness of flow cytometry. Here we describe in detail all steps in the procedure, discuss potential problems, and offer solutions.",

author = "Brussaard, {Corina P. D.} and Payet, {Jerome P.} and Christian Winter and Markus Weinbauer",

note = "Host publication data : S. W. Wilhelm, M. G. Weinbauer, and C. A. Suttle (eds.): Manual of Aquatic Viral Ecology 2010.",

year = "2010",

language = "English",

pages = "102--109",

editor = "Wilhelm, { S. W.} and { Weinbauer}, { M. G.} and { Suttle}, { C. A.}",

booktitle = "Manual of Aquatic Viral Ecology",

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TY - CHAP

T1 - Quantification of aquatic viruses by flow cytometry

AU - Brussaard, Corina P. D.

AU - Payet, Jerome P.

AU - Winter, Christian

AU - Weinbauer, Markus

N1 - Host publication data : S. W. Wilhelm, M. G. Weinbauer, and C. A. Suttle (eds.): Manual of Aquatic Viral Ecology 2010.

PY - 2010

Y1 - 2010

N2 - For many laboratories, flow cytometry is becoming the routine method for quantifying viruses in aquatic systems because of its high reproducibility, high sample throughput, and ability to distinguish several subpopulations of viruses. Comparison of viral counts between flow cytometry and epifluorescence microscopy typically shows slopes that are statistically not distinguishable from 1, thus confirming the usefulness of flow cytometry. Here we describe in detail all steps in the procedure, discuss potential problems, and offer solutions.

AB - For many laboratories, flow cytometry is becoming the routine method for quantifying viruses in aquatic systems because of its high reproducibility, high sample throughput, and ability to distinguish several subpopulations of viruses. Comparison of viral counts between flow cytometry and epifluorescence microscopy typically shows slopes that are statistically not distinguishable from 1, thus confirming the usefulness of flow cytometry. Here we describe in detail all steps in the procedure, discuss potential problems, and offer solutions.

M3 - Chapter

SP - 102

EP - 109

BT - Manual of Aquatic Viral Ecology

A2 - Wilhelm, S. W.

A2 - Weinbauer, M. G.

A2 - Suttle, C. A.

PB - Unknown publisher

ER -