Rapid, high-yield enzymatic synthesis of n.c.a. 6-[18F]fluorodopamine (6-[18F]FDA) for in vivo application

Karsten Bamminger, Julia Raitanen, Georgios Karanikas, Sazan Rasul, Lukas Nics, Markus Mitterhauser, Wolfgang Wadsak, Marcus Hacker, Verena Pichler, Chrysoula Vraka

Publications: Contribution to journalArticlePeer Reviewed

Abstract

6-[18F]Fluorodopamine (6-[18F]FDA) proved valuable as a diagnostic tool for neuroendocrine and rare tumors, such as ganglioneuromas and pheochromocytomas, however, clinical application is still limited. So far, its radiosyntheses were impeded by the formation of side-products, low molar activity, multi-step reactions and the use of challenging precursors. Here, we describe a detailed enzymatic procedure for fast, simple and high yield synthesis of no carrier added 6-[18F]FDA from 6-[18F]FDOPA with high radiochemical yield of ~69 % and >95 % purity as determined by radio-HPLC and -TLC. The product formulation was stable and suitable for in vivo application.

Original languageEnglish
Pages (from-to)189-197
Number of pages9
JournalNuclear Medicine and Biology
Volume114-115
DOIs
Publication statusPublished - 1 Nov 2022

Austrian Fields of Science 2012

  • 104020 Radiochemistry

Keywords

  • 6-FDOPA
  • 6-fluorodopamine
  • Enzymatic
  • Fluorine-18
  • Radiosynthesis
  • Tyrosine decarboxylase

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