Short-term isocaloric intake of a fructose – but not glucose-rich diet affects bacterial endotoxin concentrations and markers of metabolic health in normal weight healthy subjects

Anika Nier, Annette Brandt, Dragana Rajcic, Tony Bruns, Ina Bergheim (Corresponding author)

Publications: Contribution to journalArticlePeer Reviewed

Abstract

Scope Dietary pattern and impairments of intestinal barrier function are discussed to be critical in the development of metabolic impairments. Here, it is determined if an isocaloric exchange of complex carbohydrates with monosaccharides affects markers of intestinal permeability and metabolic health in healthy subjects. Methods and Results After a dietary standardization for 4 days, all 12 subjects aged 21-33 years receive an isocaloric fructose- and glucose-enriched diet for 3 days separated by a wash-out phase. Anthropometry, blood pressure, markers of intestinal permeability and metabolic as well as inflammatory parameters are determined in blood samples or isolated peripheral blood mononuclear cells collected at baseline, after standardizations and the monosaccharide interventions, respectively. While anthropometric and inflammatory parameters are not changed, the intake of an isocaloric fructose- but not glucose-enriched diet is associated with a significant increase of bacterial endotoxin plasma levels and alanine aminotransferase activity in serum, while total plasma nitrate/nitrite concentrations are significantly decreased. In peripheral blood mononuclear cells, Toll like receptors 4, 2, and MYD88 mRNA expressions are significantly induced after the fructose-rich but not the glucose-rich diet. Conclusion In metabolically healthy subjects, even a short-term intake of a fructose-rich diet can elevate bacterial endotoxin levels and change markers of liver health and vascular endothelial function.

Original languageEnglish
Article number1800868
Number of pages9
JournalMolecular Nutrition & Food Research
Volume63
Issue number6
DOIs
Publication statusPublished - Mar 2019

Austrian Fields of Science 2012

  • 303009 Nutritional sciences

Keywords

  • BLOOD-PRESSURE
  • ENDOTHELIAL DYSFUNCTION
  • FATTY LIVER-DISEASE
  • INDUCED STEATOSIS
  • INSULIN SENSITIVITY
  • INTESTINAL PERMEABILITY
  • LIPOPOLYSACCHARIDE-BINDING PROTEIN
  • MURINE MODEL
  • RISK-FACTORS
  • URIC-ACID
  • endothelial function
  • endotoxin
  • fructose
  • glucose
  • normal weight healthy adults

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