TY - JOUR
T1 - Simple and rapid preparation of [11C]DASB with high quality and reliability for routine applications
AU - Haeusler, Daniela
AU - Mien, Leonhard-Key
AU - Nics, Lukas
AU - Ungersböck, Johanna
AU - Philippe, Cecile
AU - Lanzenberger, Rupert
AU - Kletter, Kurt
AU - Dudczak, Robert
AU - Mitterhauser, Markus
AU - Wadsak, Wolfgang
N1 - Haeusler, D.a b , Mien, L.-K.a b , Nics, L.a c , Ungersboeck, J.a d , Philippe, C.a b , Lanzenberger, R.R.e , Kletter, K.a , Dudczak, R.a , Mitterhauser, M.a b f , Wadsak, W.a d
a Department of Nuclear Medicine, PET, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria
b Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, A-1090 Vienna, Austria
c Department of Nutritional Sciences, University of Vienna, A-1090 Vienna, Austria
d Department of Inorganic Chemistry, University of Vienna, A-1090 Vienna, Austria
e Department of Psychiatry and Psychotherapy, Medical University of Vienna, A-1090 Vienna, Austria
f Hospital Pharmacy, the General Hospital of Vienna, A-1090 Vienna, Austria
PY - 2009
Y1 - 2009
N2 - [11C]DASB combines all major prerequisites for a successful SERT-ligand, providing excellent biological properties and in-vivo behaviour. Thus, we aimed to establish a fully automated procedure for the synthesis and purification of [11C]DASB with a high degree of reliability reducing the overall synthesis time while conserving high yields and purity. The optimized [11C]DASB synthesis was applied in more than 60 applications with a very low failure rate (3.2%). We obtained yields up to 8.9 GBq (average 5.3±1.6 GBq). Radiochemical yields based on [11C]CH3I, (corrected for decay) were 66.3±6.9% with a specific radioactivity (As) of 86.8±24.3 GBq/?mol (both at the end of synthesis, EOS). Time consumption was kept to a minimum, resulting in 43 min from end of bombardment to release of the product after quality control. Form our data, it is evident that the presented method can be implemented for routine preparations of [11C]DASB with high reliability.
AB - [11C]DASB combines all major prerequisites for a successful SERT-ligand, providing excellent biological properties and in-vivo behaviour. Thus, we aimed to establish a fully automated procedure for the synthesis and purification of [11C]DASB with a high degree of reliability reducing the overall synthesis time while conserving high yields and purity. The optimized [11C]DASB synthesis was applied in more than 60 applications with a very low failure rate (3.2%). We obtained yields up to 8.9 GBq (average 5.3±1.6 GBq). Radiochemical yields based on [11C]CH3I, (corrected for decay) were 66.3±6.9% with a specific radioactivity (As) of 86.8±24.3 GBq/?mol (both at the end of synthesis, EOS). Time consumption was kept to a minimum, resulting in 43 min from end of bombardment to release of the product after quality control. Form our data, it is evident that the presented method can be implemented for routine preparations of [11C]DASB with high reliability.
U2 - 10.1016/j.apradiso.2009.03.005
DO - 10.1016/j.apradiso.2009.03.005
M3 - Article
SN - 0969-8043
VL - 67
SP - 1654
EP - 1660
JO - Applied Radiation and Isotopes
JF - Applied Radiation and Isotopes
IS - 9
ER -