TY - JOUR
T1 - Simultaneous Determination of Animal Products from Ruminant, Pig, Poultry, and Fish in Feedingstuff by Targeted High-Resolution LC-MS/MS
AU - Schlachter, Sara
AU - Tadus, Klaudia
AU - Weiss, Roland
AU - Reiter, Elisabeth
AU - Strnad, Irmengard
AU - Cichna-Markl, Margit
AU - D’Amico, Stefano
N1 - Publisher Copyright:
© 2024 The Authors. Published by American Chemical Society.
Accession Number
WOS:001259898200001
PubMed ID
38941278
PY - 2024/7/10
Y1 - 2024/7/10
N2 - The prohibition of processed animal proteins (PAPs) has been relaxed gradually since 2007. The official control method for PAPs in feedingstuff, a combination of light microscopy (LM) followed by PCR, is no longer sufficient. Thus, a targeted LC-MS/MS method was developed, which enables a tissue-specific distinction between egg and dairy products, gelatine, and PAPs derived from blood or muscle tissue of the species ruminants, pigs, poultry, and fish. Tissue-specific proteins were analyzed after tryptic digestion to peptides with high-resolution ESI-QTOF-MS. A targeted method was developed based on untargeted proteomics approaches and the selection of specific peptides (45 unique peptides in total). Proficiency testing of blank and spiked samples revealed excellent results for trueness and selectivity. Furthermore, sensitivity was achieved at a level of 0.1% (w/w) for assessed peptides. Summing up, the developed method seems to be suitable for routine analysis after verification by ring trials.
AB - The prohibition of processed animal proteins (PAPs) has been relaxed gradually since 2007. The official control method for PAPs in feedingstuff, a combination of light microscopy (LM) followed by PCR, is no longer sufficient. Thus, a targeted LC-MS/MS method was developed, which enables a tissue-specific distinction between egg and dairy products, gelatine, and PAPs derived from blood or muscle tissue of the species ruminants, pigs, poultry, and fish. Tissue-specific proteins were analyzed after tryptic digestion to peptides with high-resolution ESI-QTOF-MS. A targeted method was developed based on untargeted proteomics approaches and the selection of specific peptides (45 unique peptides in total). Proficiency testing of blank and spiked samples revealed excellent results for trueness and selectivity. Furthermore, sensitivity was achieved at a level of 0.1% (w/w) for assessed peptides. Summing up, the developed method seems to be suitable for routine analysis after verification by ring trials.
KW - high-resolution liquid chromatography mass spectrometry
KW - parallel reaction monitoring
KW - processed animal proteins
KW - proteomics
UR - http://www.scopus.com/inward/record.url?scp=85197077423&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.4c00516
DO - 10.1021/acs.jafc.4c00516
M3 - Article
AN - SCOPUS:85197077423
SN - 0021-8561
VL - 72
SP - 15357
EP - 15365
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 27
ER -