@article{3ac77009e63e4904a6e5d84959af8e47,
title = "Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy",
abstract = "High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ∼700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.",
keywords = "CAENORHABDITIS-ELEGANS, ZEBRAFISH, ILLUMINATION, CIRCUIT",
author = "Robert Prevedel and Young-Gyu Yoon and Maximilian Hoffmann and Nikita Pak and Gordon Wetzstein and Saul Kato and Tina Schr{\"o}del and Ramesh Raskar and Manuel Zimmer and Boyden, {Edward S.} and Alipasha Vaziri",
note = "Funding Information: We thank T. M{\"u}ller, P. Pasierbek, P. Forai, H. Kaplan, M. Molodtsov, K. Tessmar-Raible, F. Schlumm and Olympus Inc. for technical support and loan of equipment, as well as H. Baier (Max Planck Institute of Neurobiology) and M. Orger (Champalimaud) for sharing zebrafish lines. We thank L. Page for providing early funding for the project and D. Dalrymple for helping catalyze connections. The computational results presented have been achieved in part using the Vienna Scientific Cluster (VSC). This work was supported by the VIPS Program of the Austrian Federal Ministry of Science and Research and the City of Vienna as well as the European Commission (Marie Curie, FP7-PEOPLE-2011-IIF) (R.P.); a Samsung Scholarship (Y.-G.Y.); a US National Science Foundation (NSF) Graduate Fellowship (N.P.); the Allen Institute for Brain Science, the MIT Media Lab, the MIT McGovern Institute, US National Institutes of Health (NIH) 1R01EY023173, the MIT Synthetic Intelligence Project, the Institution of Engineering and Technology (IET) Harvey Prize, NSF CBET 1053233, the New York Stem Cell Foundation–Robertson Award, NSF CBET 1344219, NIH 1DP1NS087724, Google, the NSF Center for Brains, Minds and Machines at MIT, and Jeremy and Joyce Wertheimer (E.S.B.); the Vienna Science and Technology Fund (WWTF) project VRG10-11, Human Frontiers Science Program Project RGP0041/2012, Research Platform Quantum Phenomena and Nanoscale Biological Systems (QuNaBioS) (A.V.); and the European Community{\textquoteright}s Seventh Framework Programme/ERC no. 281869 (M.Z. and T.S.). The Institute of Molecular Pathology is funded by Boehringer Ingelheim.",
year = "2014",
month = jul,
doi = "10.1038/NMETH.2964",
language = "English",
volume = "11",
pages = "727--730",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "NATURE PUBLISHING GROUP",
number = "7",
}