TY - JOUR
T1 - StressMatic: A Novel Automated System to Induce Depressive- and Anxiety-like Phenotype in Rats
AU - Martins-Macedo, Joana
AU - Mateus-Pinheiro, António
AU - Alves, Cátia
AU - Veloso, Fernando
AU - Gomes, Eduardo D.
AU - Ribeiro, Ines
AU - Correia, Joana S.
AU - Silveira-Rosa, Tiago
AU - Alves, Nuno D.
AU - Rodrigues, Ana J.
AU - Bessa, Joao M.
AU - Sousa, Nuno
AU - Oliveira, Joao F.
AU - Patrício, Patrícia
AU - Pinto, Luísa
PY - 2023/2
Y1 - 2023/2
N2 - Major depressive disorder (MDD) is a multidimensional psychiatric disorder that is estimated to affect around 350 million people worldwide. Generating valid and effective animal models of depression is critical and has been challenging for neuroscience researchers. For preclinical studies, models based on stress exposure, such as unpredictable chronic mild stress (uCMS), are amongst the most reliable and used, despite presenting concerns related to the standardization of protocols and time consumption for operators. To overcome these issues, we developed an automated system to expose rodents to a standard uCMS protocol. Here, we compared manual (uCMS) and automated (auCMS) stress-exposure protocols. The data shows that the impact of the uCMS exposure by both methods was similar in terms of behavioral (cognition, mood, and anxiety) and physiological (cell proliferation and endocrine variations) measurements. Given the advantages of time and standardization, this automated method represents a step forward in this field of preclinical research.
AB - Major depressive disorder (MDD) is a multidimensional psychiatric disorder that is estimated to affect around 350 million people worldwide. Generating valid and effective animal models of depression is critical and has been challenging for neuroscience researchers. For preclinical studies, models based on stress exposure, such as unpredictable chronic mild stress (uCMS), are amongst the most reliable and used, despite presenting concerns related to the standardization of protocols and time consumption for operators. To overcome these issues, we developed an automated system to expose rodents to a standard uCMS protocol. Here, we compared manual (uCMS) and automated (auCMS) stress-exposure protocols. The data shows that the impact of the uCMS exposure by both methods was similar in terms of behavioral (cognition, mood, and anxiety) and physiological (cell proliferation and endocrine variations) measurements. Given the advantages of time and standardization, this automated method represents a step forward in this field of preclinical research.
KW - MIB
KW - automated rack
KW - stress-exposure
KW - protocols standardization
KW - preclinical research
UR - http://www.scopus.com/inward/record.url?scp=85147869597&partnerID=8YFLogxK
U2 - 10.3390/cells12030381
DO - 10.3390/cells12030381
M3 - Article
SN - 2073-4409
VL - 12
JO - Cells
JF - Cells
IS - 3
M1 - 381
ER -