TY - JOUR
T1 - Sympathetic nerve innervation and metabolism in ischemic myocardium in response to remote ischemic perconditioning
AU - Kiss, Attila
AU - Wu, Ping
AU - Schlederer, Michaela
AU - Pilz, Patrick M.
AU - Szabo, Petra Lujza
AU - Li, Jingle
AU - Weber, Lukas
AU - Vraka, Chrysoula
AU - Pichler, Verena
AU - Mitterhauser, Markus
AU - Zhang, Xiaoli
AU - Zins, Karin
AU - Abraham, Dietmar
AU - Li, Sijin
AU - Podesser, Bruno K.
AU - Hacker, Marcus
AU - Li, Xiang
N1 - Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Sympathetic nerve denervation after myocardial infarction (MI) predicts risk of sudden cardiac death. Therefore, therapeutic approaches limit infarct size, improving adverse remodeling and restores sympathetic innervation have a great clinical potential. Remote ischemic perconditioning (RIPerc) could markedly attenuate MI-reperfusion (MIR) injury. In this study, we aimed to assess its effects on cardiac sympathetic innervation and metabolism. Transient myocardial ischemia is induced by ligature of the left anterior descending coronary artery (LAD) in male Sprague-Dawley rats, and in vivo cardiac 2-[F-18]FDG and [C-11]mHED PET scans were performed at 14-15 days after ischemia. RIPerc was induced by three cycles of 5-min-long unilateral hind limb ischemia and intermittent 5 min of reperfusion during LAD occlusion period. The PET quantitative parameters were quantified in parametric polar maps. This standardized format facilitates the regional radioactive quantification in deficit regions to remote areas. The ex vivo radionuclide distribution was additionally identified using autoradiography. Myocardial neuron density (tyrosine hydroxylase positive staining) and chondroitin sulfate proteoglycans (CSPG, inhibiting neuron regeneration) expression were assessed by immunohistochemistry. There was no significant difference in the mean hypometabolism 2-[F-18]FDG uptake ratio (44.6 +/- 4.8% vs. 45.4 +/- 4.4%) between MIR rats and MIR + RIPerc rats (P > 0.05). However, the mean [C-11]mHED nervous activity of denervated myocardium was significantly elevated in MIR + RIPerc rats compared to the MIR rats (35.9 +/- 7.1% vs. 28.9 +/- 2.3%, P < 0.05), coupled with reduced denervated myocardium area (19.5 +/- 5.3% vs. 27.8 +/- 6.6%, P < 0.05), which were associated with preserved left-ventricular systolic function, a less reduction in neuron density, and a significant reduction in CSPG and CD68 expression in the myocardium. RIPerc presented a positive effect on cardiac sympathetic-nerve innervation following ischemia, but showed no significant effect on myocardial metabolism.
AB - Sympathetic nerve denervation after myocardial infarction (MI) predicts risk of sudden cardiac death. Therefore, therapeutic approaches limit infarct size, improving adverse remodeling and restores sympathetic innervation have a great clinical potential. Remote ischemic perconditioning (RIPerc) could markedly attenuate MI-reperfusion (MIR) injury. In this study, we aimed to assess its effects on cardiac sympathetic innervation and metabolism. Transient myocardial ischemia is induced by ligature of the left anterior descending coronary artery (LAD) in male Sprague-Dawley rats, and in vivo cardiac 2-[F-18]FDG and [C-11]mHED PET scans were performed at 14-15 days after ischemia. RIPerc was induced by three cycles of 5-min-long unilateral hind limb ischemia and intermittent 5 min of reperfusion during LAD occlusion period. The PET quantitative parameters were quantified in parametric polar maps. This standardized format facilitates the regional radioactive quantification in deficit regions to remote areas. The ex vivo radionuclide distribution was additionally identified using autoradiography. Myocardial neuron density (tyrosine hydroxylase positive staining) and chondroitin sulfate proteoglycans (CSPG, inhibiting neuron regeneration) expression were assessed by immunohistochemistry. There was no significant difference in the mean hypometabolism 2-[F-18]FDG uptake ratio (44.6 +/- 4.8% vs. 45.4 +/- 4.4%) between MIR rats and MIR + RIPerc rats (P > 0.05). However, the mean [C-11]mHED nervous activity of denervated myocardium was significantly elevated in MIR + RIPerc rats compared to the MIR rats (35.9 +/- 7.1% vs. 28.9 +/- 2.3%, P < 0.05), coupled with reduced denervated myocardium area (19.5 +/- 5.3% vs. 27.8 +/- 6.6%, P < 0.05), which were associated with preserved left-ventricular systolic function, a less reduction in neuron density, and a significant reduction in CSPG and CD68 expression in the myocardium. RIPerc presented a positive effect on cardiac sympathetic-nerve innervation following ischemia, but showed no significant effect on myocardial metabolism.
KW - Myocardial ischemia
KW - reperfusion
KW - Remote ischemic perconditioning
KW - Cardiac sympathetic nerve
KW - Cardiac metabolism
KW - HEART
KW - DENERVATION
KW - VASOCONSTRICTION
KW - INFARCTION
KW - SYSTEM
KW - Myocardial ischemia/reperfusion
UR - http://www.scopus.com/inward/record.url?scp=85136848806&partnerID=8YFLogxK
U2 - 10.1007/s00395-022-00946-3
DO - 10.1007/s00395-022-00946-3
M3 - Article
SN - 0300-8428
VL - 117
JO - Basic research in cardiology
JF - Basic research in cardiology
IS - 1
M1 - 42
ER -