@inbook{9428ef14e94e4bc5a51cbf7ad25ef41b,
title = "Synthesis of polyethylenimine-based nanocarriers for systemic tumor targeting of nucleic acids",
abstract = "Nucleic acid-based therapies offer the option to treat tumors in a highly selective way, while toxicity towards healthy tissue can be avoided when proper delivery vehicles are used. We have recently developed carrier systems based on linear polyethylenimine, which after chemical coupling of protein- or peptide-based ligands can form nanosized polyplexes with plasmid DNA (pDNA) or RNA and deliver their payload into target cells by receptor-mediated endocytosis. This chapter describes the synthesis of LPEI from a precursor polymer and the current coupling techniques and purification procedure for peptide conjugates with linear polyethylenimine. A protocol is also given for the formation and characterization of polyplexes formed with LPEI conjugate and pDNA.",
keywords = "EGF receptor, Gene delivery, Molecular conjugates, Polyethylene glycol, Polyethylenimine, Targeting",
author = "Wolfgang R{\"o}dl and Alexander Taschauer and David Schaffert and Ernst Wagner and Manfred Ogris",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-9092-4_6",
language = "English",
isbn = "978-1-4939-9091-7",
series = "Methods in Molecular Biology",
publisher = "Humana Press, Inc.",
pages = "83--99",
booktitle = "Methods in Molecular Biology",
edition = "2",
}