Targeted Analysis of Chromatin Events (TACE)

Jason Sims, Changbin Chen, Peter Schlögelhofer, Marie-Therese Kurzbauer (Corresponding author)

Publications: Contribution to bookChapterPeer Reviewed

Abstract

Visualization of meiotic chromatin from pollen mother cells has become an essential technique to study meiosis in the model plant Arabidopsis thaliana. Here we present an advanced cytogenetic method that combines improved immunocytology with chromosome painting, thereby generating a tool to quantitatively analyze localization of proteins to any given genomic region. Proteins involved in different processes such as DNA double-strand break formation and recombinational repair can be visualized on meiotic chromatin with the additional feature of assessing their abundance at specific chromosomal locations.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages47-58
Number of pages12
Volume2061
DOIs
Publication statusPublished - 2020

Publication series

SeriesMethods in Molecular Biology
ISSN1064-3745

Austrian Fields of Science 2012

  • 106013 Genetics
  • 106023 Molecular biology

Keywords

  • Arabidopsis thaliana
  • Chromosome painting
  • FISH
  • Immunocytology
  • Meiosis

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