The ATP-binding cassette (ABC) transporter Bpt1p mediates vacuolar sequestration of glutathione conjugates in yeast

Markus Klein; Yasmine M. Mamnun; Thomas Eggmann; Christoph Schüller; Enrico Martinoia; Karl Kuchler

The ATP-binding cassette (ABC) transporter Bpt1p mediates vacuolar sequestration of glutathione conjugates in yeast

Markus Klein, Yasmine M. Mamnun, Thomas Eggmann, Christoph Schüller, Enrico Martinoia, Karl Kuchler

Publications: Contribution to journal › Article › Peer Reviewed

Abstract

Vacuolar sequestration or cellular extrusion of glutathione-conjugated xenobiotics and catabolites by ATP-binding cassette (ABC) transporters is an important detoxification mechanism operating in many species. In this study, we show that the yeast ABC transporter Bpt1p, a paralogue of Ycf1p, acts as an ATP-dependent vacuolar pump for glutathione conjugates. Bpt1p, which is inhibited by vanadate and glibenclamide, accounts for one third of the total vacuolar transport of glutathione conjugates. Furthermore, immunoblot analyses show that Bpt1p levels are strongly elevated in early stationary phase, consistent with a function of Bpt1p in vacuolar detoxification. Œ 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	63-67
Number of pages	5
Journal	FEBS Letters
Volume	520
Issue number	1-3
Publication status	Published - 2002

Austrian Fields of Science 2012

1060 Biology

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Klein, M., Mamnun, Y. M., Eggmann, T., Schüller, C., Martinoia, E., & Kuchler, K. (2002). The ATP-binding cassette (ABC) transporter Bpt1p mediates vacuolar sequestration of glutathione conjugates in yeast. FEBS Letters, 520(1-3), 63-67. http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-45RFR2X-2&_user=464575&_coverDate=06%2F05%2F2002&_rdoc=13&_fmt=summary&_orig=browse&_srch=doc-info(%23toc%234938%232002%23994799998%23318632%23FLA%23display%23Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=39&_acct=C000022258&_version=1&_urlVersion=0&_userid=464575&md5=af1f3a4ad59b037964cbf087ee43227f

@article{79cbc5f93c70429189fb4367d5bb2b25,

title = "The ATP-binding cassette (ABC) transporter Bpt1p mediates vacuolar sequestration of glutathione conjugates in yeast",

abstract = "Vacuolar sequestration or cellular extrusion of glutathione-conjugated xenobiotics and catabolites by ATP-binding cassette (ABC) transporters is an important detoxification mechanism operating in many species. In this study, we show that the yeast ABC transporter Bpt1p, a paralogue of Ycf1p, acts as an ATP-dependent vacuolar pump for glutathione conjugates. Bpt1p, which is inhibited by vanadate and glibenclamide, accounts for one third of the total vacuolar transport of glutathione conjugates. Furthermore, immunoblot analyses show that Bpt1p levels are strongly elevated in early stationary phase, consistent with a function of Bpt1p in vacuolar detoxification. {\OE} 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.",

author = "Markus Klein and Mamnun, {Yasmine M.} and Thomas Eggmann and Christoph Sch{\"u}ller and Enrico Martinoia and Karl Kuchler",

note = " DOI: 10.1016/S0014-5793(02)02767-9 Coden: FEBLA Affiliations: Institute of Medical Biochemistry, University of Vienna, Vienna BioCenter, Dr. Bohr-Gasse 9/2, A-1030 Vienna, Austria Adressen: Kuchler, K.; Institute of Medical Biochemistry; University of Vienna; Vienna BioCenter; Dr. Bohr-Gasse 9/2 A-1030 Vienna, Austria; email: [email protected] Source-File: MFPLUniWienScopus.csv Import aus Scopus: 2-s2.0-0037024336 Importdatum: 07.12.2006 15:17:06 15.01.2009: Datenanforderung 2652 (Import Sachbearbeiter) 09.02.2010: Datenanforderung UNIVIS-DATEN-DAT.RA-2 (Import Sachbearbeiter)",

year = "2002",

language = "English",

volume = "520",

pages = "63--67",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "1-3",

}

Klein, M, Mamnun, YM, Eggmann, T, Schüller, C, Martinoia, E & Kuchler, K 2002, 'The ATP-binding cassette (ABC) transporter Bpt1p mediates vacuolar sequestration of glutathione conjugates in yeast', FEBS Letters, vol. 520, no. 1-3, pp. 63-67. <http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-45RFR2X-2&_user=464575&_coverDate=06%2F05%2F2002&_rdoc=13&_fmt=summary&_orig=browse&_srch=doc-info(%23toc%234938%232002%23994799998%23318632%23FLA%23display%23Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=39&_acct=C000022258&_version=1&_urlVersion=0&_userid=464575&md5=af1f3a4ad59b037964cbf087ee43227f>

TY - JOUR

T1 - The ATP-binding cassette (ABC) transporter Bpt1p mediates vacuolar sequestration of glutathione conjugates in yeast

AU - Klein, Markus

AU - Mamnun, Yasmine M.

AU - Eggmann, Thomas

AU - Schüller, Christoph

AU - Martinoia, Enrico

AU - Kuchler, Karl

N1 - DOI: 10.1016/S0014-5793(02)02767-9 Coden: FEBLA Affiliations: Institute of Medical Biochemistry, University of Vienna, Vienna BioCenter, Dr. Bohr-Gasse 9/2, A-1030 Vienna, Austria Adressen: Kuchler, K.; Institute of Medical Biochemistry; University of Vienna; Vienna BioCenter; Dr. Bohr-Gasse 9/2 A-1030 Vienna, Austria; email: [email protected] Source-File: MFPLUniWienScopus.csv Import aus Scopus: 2-s2.0-0037024336 Importdatum: 07.12.2006 15:17:06 15.01.2009: Datenanforderung 2652 (Import Sachbearbeiter) 09.02.2010: Datenanforderung UNIVIS-DATEN-DAT.RA-2 (Import Sachbearbeiter)

PY - 2002

Y1 - 2002

N2 - Vacuolar sequestration or cellular extrusion of glutathione-conjugated xenobiotics and catabolites by ATP-binding cassette (ABC) transporters is an important detoxification mechanism operating in many species. In this study, we show that the yeast ABC transporter Bpt1p, a paralogue of Ycf1p, acts as an ATP-dependent vacuolar pump for glutathione conjugates. Bpt1p, which is inhibited by vanadate and glibenclamide, accounts for one third of the total vacuolar transport of glutathione conjugates. Furthermore, immunoblot analyses show that Bpt1p levels are strongly elevated in early stationary phase, consistent with a function of Bpt1p in vacuolar detoxification. Œ 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

AB - Vacuolar sequestration or cellular extrusion of glutathione-conjugated xenobiotics and catabolites by ATP-binding cassette (ABC) transporters is an important detoxification mechanism operating in many species. In this study, we show that the yeast ABC transporter Bpt1p, a paralogue of Ycf1p, acts as an ATP-dependent vacuolar pump for glutathione conjugates. Bpt1p, which is inhibited by vanadate and glibenclamide, accounts for one third of the total vacuolar transport of glutathione conjugates. Furthermore, immunoblot analyses show that Bpt1p levels are strongly elevated in early stationary phase, consistent with a function of Bpt1p in vacuolar detoxification. Œ 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

M3 - Article

SN - 0014-5793

VL - 520

SP - 63

EP - 67

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

The ATP-binding cassette (ABC) transporter Bpt1p mediates vacuolar sequestration of glutathione conjugates in yeast

Abstract

Austrian Fields of Science 2012

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