The endexine: a frequently overlooked pollen wall layer and a simple method for detection

Martina Weber (Corresponding author), Silvia Ulrich

    Publications: Contribution to journalArticlePeer Reviewed

    Abstract

    For the detection of pollen wall layers, the use of different staining methods for one and the same species is highly recommended. The usage of standard transmission electron microscopy (TEM) staining methods showed that the ektexine-layers have always the same contrast behaviour, while the endexine changes its electron opaqueness depending on the method used. However, the endexine can often not be discriminated from the other wall layers. A simple method to detect the endexine is the use of potassium permanganate, which stains the layer electron dense, producing a distinct contrast.
    Original languageEnglish
    Pages (from-to)83-90
    Number of pages8
    JournalGrana: an international journal of palynology and aerobiology with world pollen and spore flora
    Volume49
    Issue number2
    DOIs
    Publication statusPublished - 2010

    Austrian Fields of Science 2012

    • 1060 Biology

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