TY - JOUR
T1 - The FinO/ProQ-like protein PA2582 impacts antimicrobial resistance in Pseudomonas aeruginosa
AU - Cianciulli Sesso, Anastasia
AU - Resch, Armin
AU - Moll, Isabella
AU - Bläsi, Udo
AU - Sonnleitner, Elisabeth
N1 - Funding Information:
The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. The work was supported by the Austrian Science Fund (www.fwf.ac.at/en) through project P33617-B (UB and ES). AC was supported through the FWF funded doctoral program RNA-Biology W-1207.
Publisher Copyright:
Copyright © 2024 Cianciulli Sesso, Resch, Moll, Bläsi and Sonnleitner.
PY - 2024/6/26
Y1 - 2024/6/26
N2 - Bacteria employ small regulatory RNAs (sRNA) and/or RNA binding proteins (RBPs) to respond to environmental cues. In Enterobacteriaceae, the FinO-domain containing RBP ProQ associates with numerous sRNAs and mRNAs, impacts sRNA-mediated riboregulation or mRNA stability by binding to 5′- or 3′-untranslated regions as well as to internal stem loop structures. Global RNA-protein interaction studies and sequence comparisons identified a ProQ-like homolog (PA2582/ProQPae) in Pseudomonas aeruginosa (Pae). To address the function of ProQPae, at first a comparative transcriptome analysis of the Pae strains PAO1 and PAO1ΔproQ was performed. This study revealed more than 100 differentially abundant transcripts, affecting a variety of cellular functions. Among these transcripts were pprA and pprB, encoding the PprA/PprB two component system, psrA, encoding a transcriptional activator of pprB, and oprI, encoding the outer membrane protein OprI. RNA co-purification experiments with Strep-tagged Pae ProQ protein corroborated an association of ProQPae with these transcripts. In accordance with the up-regulation of the psrA, pprA, and pprB genes in strain PAO1ΔproQ a phenotypic analysis revealed an increased susceptibility toward the aminoglycosides tobramycin and gentamicin in biofilms. Conversely, the observed down-regulation of the oprI gene in PAO1ΔproQ could be reconciled with a decreased susceptibility toward the synthetic cationic antimicrobial peptide GW-Q6. Taken together, these studies revealed that ProQPae is an RBP that impacts antimicrobial resistance in Pae.
AB - Bacteria employ small regulatory RNAs (sRNA) and/or RNA binding proteins (RBPs) to respond to environmental cues. In Enterobacteriaceae, the FinO-domain containing RBP ProQ associates with numerous sRNAs and mRNAs, impacts sRNA-mediated riboregulation or mRNA stability by binding to 5′- or 3′-untranslated regions as well as to internal stem loop structures. Global RNA-protein interaction studies and sequence comparisons identified a ProQ-like homolog (PA2582/ProQPae) in Pseudomonas aeruginosa (Pae). To address the function of ProQPae, at first a comparative transcriptome analysis of the Pae strains PAO1 and PAO1ΔproQ was performed. This study revealed more than 100 differentially abundant transcripts, affecting a variety of cellular functions. Among these transcripts were pprA and pprB, encoding the PprA/PprB two component system, psrA, encoding a transcriptional activator of pprB, and oprI, encoding the outer membrane protein OprI. RNA co-purification experiments with Strep-tagged Pae ProQ protein corroborated an association of ProQPae with these transcripts. In accordance with the up-regulation of the psrA, pprA, and pprB genes in strain PAO1ΔproQ a phenotypic analysis revealed an increased susceptibility toward the aminoglycosides tobramycin and gentamicin in biofilms. Conversely, the observed down-regulation of the oprI gene in PAO1ΔproQ could be reconciled with a decreased susceptibility toward the synthetic cationic antimicrobial peptide GW-Q6. Taken together, these studies revealed that ProQPae is an RBP that impacts antimicrobial resistance in Pae.
KW - aminoglycosides
KW - antibiotic resistance
KW - antimicrobial peptides
KW - ProQ
KW - Pseudomonas aeruginosa
KW - RNA-binding protein
UR - http://www.scopus.com/inward/record.url?scp=85198534164&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2024.1422742
DO - 10.3389/fmicb.2024.1422742
M3 - Article
AN - SCOPUS:85198534164
SN - 1664-302X
VL - 15
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 1422742
ER -