The linear Streptomyces plasmid pBL1: analyses of transfer functions

Sergey B. Zotchev; Hildgund Schrempf

doi:10.1007/BF00281786

The linear Streptomyces plasmid pBL1: analyses of transfer functions

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Abstract

pBLI is a conjugative linear extrachromosomal element of 43 kb previously isolated after interspecific mating between Streptomyces bambergiensis and S. lividans. Cloning experiments using the non-conjugative, circular Streptomyces vector pIJ702 allowed the identification of a 5.74 kb region from pBL1 which facilitates plasmid transfer. Insertion and deletion mutagenesis, gene disruptions, and sequence data suggest that at least five previously unknown genes of pBL1 are required for efficient plasmid transfer and its regulation.

Original language	English
Pages (from-to)	374-382
Number of pages	9
Journal	Molecular and general genetics : MGG
Volume	242
Issue number	4
DOIs	https://doi.org/10.1007/BF00281786
Publication status	Published - Feb 1994

Austrian Fields of Science 2012

106013 Genetics
106022 Microbiology

Keywords

Nucleotide sequence
Plasmid
Streptomyces lividans
Transfer genes

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10.1007/BF00281786

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abstract = "pBLI is a conjugative linear extrachromosomal element of 43 kb previously isolated after interspecific mating between Streptomyces bambergiensis and S. lividans. Cloning experiments using the non-conjugative, circular Streptomyces vector pIJ702 allowed the identification of a 5.74 kb region from pBL1 which facilitates plasmid transfer. Insertion and deletion mutagenesis, gene disruptions, and sequence data suggest that at least five previously unknown genes of pBL1 are required for efficient plasmid transfer and its regulation.",

keywords = "Nucleotide sequence, Plasmid, Streptomyces lividans, Transfer genes",

author = "Zotchev, {Sergey B.} and Hildgund Schrempf",

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AU - Zotchev, Sergey B.

AU - Schrempf, Hildgund

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AB - pBLI is a conjugative linear extrachromosomal element of 43 kb previously isolated after interspecific mating between Streptomyces bambergiensis and S. lividans. Cloning experiments using the non-conjugative, circular Streptomyces vector pIJ702 allowed the identification of a 5.74 kb region from pBL1 which facilitates plasmid transfer. Insertion and deletion mutagenesis, gene disruptions, and sequence data suggest that at least five previously unknown genes of pBL1 are required for efficient plasmid transfer and its regulation.

KW - Nucleotide sequence

KW - Plasmid

KW - Streptomyces lividans

KW - Transfer genes

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DO - 10.1007/BF00281786

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JO - Molecular and general genetics : MGG

JF - Molecular and general genetics : MGG

IS - 4

ER -

The linear Streptomyces plasmid pBL1: analyses of transfer functions

Abstract

Austrian Fields of Science 2012

Keywords

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