Abstract
An increased conversion of white adipocytes to a “brite” adipocyte phenotype, characterized by increased number of mitochondria and increased expression of uncoupling protein 1 (UPC-1), represents a promising anti-obesity approach. Beside an activation of beta-adrenergic signalling by a cold ambient temperature, also the activation of several temperature-sensing transient receptor potential (TRP) ion channels has been associated with browning of white adipocytes. In the present study, we hypothesized that the two food-derived aroma compounds and TRP-channel agonists nonivamide and cinnamaldehyde augment the thermogenic response of 3T3-L1 white adipocytes to a cold stimulus. We found that upon incubation at 29°C, 3T3-L1 adipocytes show an increased expressionof UCP-1 at the levels of gene transcripts and protein level as well as increased mitochondrial biogenesis in comparison to 37°C, confirming the validity of the cellular model. In addition, we demonstrate here that treatment of 3T3-L1 cells with 0.1μM nonivamide or 10μM cinnamaldehyde for 48 h increased mitochondrial biogenesis when incubated at 29°C, but not at 37°C. These data hint towards beneficial effects of the two aroma compounds when applied as a chronic treatment in addition to a cold stimulus, which has to be validated in future studies.
Original language | English |
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Title of host publication | Flavour Science : Proceedings of the XV Weurman Flavour Research Symposium |
Subtitle of host publication | 18.-22. September 2017, Graz University of Technology, Austria |
Editors | Barbara Siegmund, Erich Leitner |
Place of Publication | Graz |
Publisher | Verlag der Technischen Universität Graz |
Pages | 273 – 280 |
Edition | 1 |
ISBN (Print) | 978-3-85125-593-5 |
DOIs | |
Publication status | Published - Aug 2018 |
Event | 15th Weurman Flavour Research Symposium - Technische Universität Graz, Graz, Austria Duration: 18 Sept 2017 → 22 Sept 2017 http://www.analytchem.tugraz.at/weurman/ |
Conference
Conference | 15th Weurman Flavour Research Symposium |
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Country/Territory | Austria |
City | Graz |
Period | 18/09/17 → 22/09/17 |
Internet address |
Austrian Fields of Science 2012
- 303009 Nutritional sciences