The TRP channel agonists nonivamide and cinnamaldehyde augment cold-induced mitochondrial biogenesis in white adipocytes

Barbara Lieder, Kerstin Schweiger, Veronika Somoza

Publications: Contribution to bookContribution to proceedingsPeer Reviewed

Abstract

An increased conversion of white adipocytes to a “brite” adipocyte phenotype, characterized by increased number of mitochondria and increased expression of uncoupling protein 1 (UPC-1), represents a promising anti-obesity approach. Beside an activation of beta-adrenergic signalling by a cold ambient temperature, also the activation of several temperature-sensing transient receptor potential (TRP) ion channels has been associated with browning of white adipocytes. In the present study, we hypothesized that the two food-derived aroma compounds and TRP-channel agonists nonivamide and cinnamaldehyde augment the thermogenic response of 3T3-L1 white adipocytes to a cold stimulus. We found that upon incubation at 29°C, 3T3-L1 adipocytes show an increased expressionof UCP-1 at the levels of gene transcripts and protein level as well as increased mitochondrial biogenesis in comparison to 37°C, confirming the validity of the cellular model. In addition, we demonstrate here that treatment of 3T3-L1 cells with 0.1μM nonivamide or 10μM cinnamaldehyde for 48 h increased mitochondrial biogenesis when incubated at 29°C, but not at 37°C. These data hint towards beneficial effects of the two aroma compounds when applied as a chronic treatment in addition to a cold stimulus, which has to be validated in future studies.
Original languageEnglish
Title of host publicationFlavour Science : Proceedings of the XV Weurman Flavour Research Symposium
Subtitle of host publication18.-22. September 2017, Graz University of Technology, Austria
EditorsBarbara Siegmund, Erich Leitner
Place of PublicationGraz
PublisherVerlag der Technischen Universität Graz
Pages273 – 280
Edition1
ISBN (Print)978-3-85125-593-5
DOIs
Publication statusPublished - Aug 2018
Event15th Weurman Flavour Research Symposium - Technische Universität Graz, Graz, Austria
Duration: 18 Sept 201722 Sept 2017
http://www.analytchem.tugraz.at/weurman/

Conference

Conference15th Weurman Flavour Research Symposium
Country/TerritoryAustria
CityGraz
Period18/09/1722/09/17
Internet address

Austrian Fields of Science 2012

  • 303009 Nutritional sciences

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