Uptake of diet resveratrol into the human low-density lipoprotein. Identification and quantification of resveratrol metabolites by liquid chromatography coupled with tandem mass spectrometry

Mireia Urpi-Sarda, Olga Jauregui, Rosa M. Lamuela-Raventos, Walter Jäger, Michaela Böhmdorfer, Maria-Isabel Covas, Cristina Andres-Lacueva

    Publications: Contribution to journalArticlePeer Reviewed

    Abstract

    In this paper, a sensitive, precise, and selective analytical method has been developed for the identification and quantification of resveratrol metabolites in human low-density lipoprotein (IDL) after moderate consumption of red wine, using high-performance liquid chromatography electrospray in tandem mass spectrometry (LC-ESI-MS/ MS). From different extraction procedures tested, solid-phase extraction was selected to minimize matrix effects reaching the highest sensitivity. Standard calibration curves prepared in human LDL for trans-resveratrol were linear over a range of 0.44-438.59 pmol/mL. The accuracy and interassay precision of this LC-MS/MS assay for resveratrol showed a coefficient of variation of
    Original languageEnglish
    Pages (from-to)3149-3155
    Number of pages7
    JournalAnalytical Chemistry
    Volume77
    Issue number10
    Publication statusPublished - 2005

    Austrian Fields of Science 2012

    • 3012 Pharmacy, Pharmacology, Toxicology

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